Pleiotropic role of Rac in mast cell activation revealed by a cell permeable Bordetella dermonecrotic fusion toxin.

Authors :: Stratmann H
Schwan C
Orth JH
Schmidt G
Aktories K
Source :: Cellular signalling [Cell Signal] 2010 Jul; Vol. 22 (7), pp. 1124-31. Date of Electronic Publication: 2010 Mar 06.
Publication Year :: 2010
Abstract: To activate the GTPase Rac in rat basophilic leukemia (RBL) cells and mouse bone marrow-derived mast cells (BMMC) a TAT fusion toxin of Bordetella dermonecrotic toxin (DNT-TAT) was constructed. The fusion toxin activated Rac1 and RhoA in vitro but only Rac in RBL cells and BMMC. DNT-TAT caused an increase in inositol phosphate formation, calcium mobilization, ERK activation and degranulation of mast cells. All these effects were inhibited by the Rho GTPase-inactivating Clostridium difficile toxin B and Clostridium sordellii lethal toxin. Also the calcium ionophore A23187 caused mast cell activation, including ERK phosphorylation, by processes involving an activation of Rac. The data indicate pleiotropic functions of Rac in mast cell activation.<br /> ((c) 2010 Elsevier Inc. All rights reserved.)

Full Text Access

Tools