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Human intestinal MUC17 mucin augments intestinal cell restitution and enhances healing of experimental colitis.
- Source :
-
The international journal of biochemistry & cell biology [Int J Biochem Cell Biol] 2010 Jun; Vol. 42 (6), pp. 996-1006. Date of Electronic Publication: 2010 Mar 06. - Publication Year :
- 2010
-
Abstract
- Unlabelled: The membrane-bound mucins, MUC17 (human) and Muc3 (mouse), are highly expressed on the apical surface of intestinal epithelia and are thought to be cytoprotective. The extracellular regions of these mucins contain EGF-like Cys-rich segments (CRD1 and CRD2) connected by an intervening linker domain (L). The purpose of this study was to determine the functional activity of human MUC17 membrane-bound mucin.<br />Methods: Endogenous MUC17 was inhibited in LS174T colon cells by stable transfection of a small hairpin RNA targeting MUC17 (LSsi cells). The effect of recombinant MUC17-CRD1-L-CRD2 protein on migration, apoptosis, and experimental colitis was determined.<br />Results: Reduced MUC17 expression in LSsi cells was associated with visibly reduced cell aggregation, reduced cell-cell adherence, and reduced cell migration, but no change in tumorigenicity. LSsi cells also demonstrated a 3.7-fold increase in apoptosis rates compared with control cells following treatment with etoposide. Exposure of colonic cell lines to exogenous recombinant MUC17-CRD1-L-CRD2 protein significantly increased cell migration and inhibited apoptosis. As a marker of biologic activity, MUC17-CRD1-L-CRD2 proteins stimulate ERK phosphorylation in colonic cell lines; and inhibition of ERK phosphorylation reduced the anti-apoptosis and migratory effect of MUC17-CRD1-L-CRD2. Finally, mice treated with MUC17-CRD1-L-CRD2 protein given per rectum demonstrated accelerated healing in acetic acid and dextran sodium sulfate induced colitis in vivo. These data indicate that both native MUC17 and the exogenous recombinant cysteine-rich domain of MUC17 play a role in diverse cellular mechanisms related to cell restitution, and suggest a potential role for MUC17-CRD1-L-CRD2 recombinant protein in the treatment of mucosal inflammatory diseases.<br /> (Published by Elsevier Ltd.)
- Subjects :
- Animals
Apoptosis drug effects
Apoptosis genetics
Cell Adhesion drug effects
Cell Adhesion genetics
Cell Aggregation drug effects
Cell Line, Tumor
Cell Proliferation drug effects
Colitis drug therapy
Colitis pathology
Colonic Neoplasms pathology
Female
Humans
Intestinal Mucosa drug effects
Intestinal Mucosa pathology
Mice
Mice, Nude
Models, Animal
Mucins genetics
Mucins pharmacology
Neoplasm Transplantation
Protein Engineering
RNA, Small Interfering genetics
Recombinant Fusion Proteins genetics
Recombinant Fusion Proteins pharmacology
Wound Healing drug effects
Colitis metabolism
Colonic Neoplasms metabolism
Intestinal Mucosa metabolism
Mucins metabolism
Recombinant Fusion Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5875
- Volume :
- 42
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- The international journal of biochemistry & cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 20211273
- Full Text :
- https://doi.org/10.1016/j.biocel.2010.03.001