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Ischemic postconditioning does not attenuate ischemia-reperfusion injury of rabbit small intestine.
- Source :
-
Veterinary surgery : VS [Vet Surg] 2010 Feb; Vol. 39 (2), pp. 216-23. - Publication Year :
- 2010
-
Abstract
- Objective: To determine whether ischemic postconditioning can attenuate intestinal ischemia-reperfusion (I-R) injury and has a beneficial effect on tissue blood flow during reperfusion.<br />Study Design: In vivo experimental study.<br />Animals: New Zealand White rabbits (n=6).<br />Methods: Rabbits were anesthetized with pentobarbital, to avoid the preconditioning effects of volatile anesthetics, and ventilated with room air. Rectal temperature, hemodynamics, and normocapnia were maintained. After celiotomy, 3 jejunal segments were isolated in each rabbit for the following groups: (1) control, (2) I-R, and (3) I-R with postconditioning. I-R was induced by a 45-minute occlusion of the segment jejunal artery followed by 2-hour reperfusion. The postconditioning segment had 4 cycles of 30-second reperfusion and 30-second reocclusion during the initial 4 minutes of reperfusion. Stable isotope-labeled microspheres were used to measure intestinal blood flow at baseline, end occlusion, and end reperfusion. At the end of reperfusion, intestine segments were harvested and the rabbits euthanatized. A semiquantitative histopathologic evaluation (0-5) was conducted by a single, blinded observer. Wet-to-dry weight ratios were calculated to assess intestinal edema.<br />Results: There was no significant difference in grade of necrosis, tissue wet-to-dry weight ratios, or blood flow at any time point between ischemic and postconditioning groups.<br />Conclusions: Ischemic postconditioning was ineffective in this model of intestinal I-R.<br />Clinical Relevance: Further experimental studies will need to be performed before clinical application of postconditioning for intestinal ischemia.
- Subjects :
- Animals
Hemodynamics
Jejunal Diseases pathology
Jejunal Diseases prevention & control
Jejunal Diseases veterinary
Rabbits
Regional Blood Flow
Reperfusion Injury pathology
Reperfusion Injury prevention & control
Intestine, Small blood supply
Intestine, Small pathology
Ischemic Preconditioning veterinary
Reperfusion Injury veterinary
Subjects
Details
- Language :
- English
- ISSN :
- 1532-950X
- Volume :
- 39
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Veterinary surgery : VS
- Publication Type :
- Academic Journal
- Accession number :
- 20210969
- Full Text :
- https://doi.org/10.1111/j.1532-950X.2009.00619.x