Back to Search
Start Over
Loss of the actin remodeler Eps8 causes intestinal defects and improved metabolic status in mice.
- Source :
-
PloS one [PLoS One] 2010 Mar 02; Vol. 5 (3), pp. e9468. Date of Electronic Publication: 2010 Mar 02. - Publication Year :
- 2010
-
Abstract
- Background: In a variety of organisms, including mammals, caloric restriction improves metabolic status and lowers the incidence of chronic-degenerative diseases, ultimately leading to increased lifespan.<br />Methodology/principal Findings: Here we show that knockout mice for Eps8, a regulator of actin dynamics, display reduced body weight, partial resistance to age- or diet-induced obesity, and overall improved metabolic status. Alteration in the liver gene expression profile, in behavior and metabolism point to a calorie restriction-like phenotype in Eps8 knockout mice. Additionally, and consistent with a calorie restricted metabolism, Eps8 knockout mice show increased lifespan. The metabolic alterations in Eps8 knockout mice correlated with a significant reduction in intestinal fat absorption presumably caused by a 25% reduction in intestinal microvilli length.<br />Conclusions/significance: Our findings implicate actin dynamics as a novel variable in the determination of longevity. Additionally, our observations suggest that subtle differences in energy balance can, over time, significantly affect bodyweight and metabolic status in mice.
- Subjects :
- 3T3-L1 Cells
Adaptor Proteins, Signal Transducing genetics
Animals
Body Weight
Caco-2 Cells
Caloric Restriction
Cytoskeletal Proteins genetics
Energy Metabolism
Female
Gene Expression Profiling
Gene Expression Regulation
Humans
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Microvilli metabolism
Actins metabolism
Adaptor Proteins, Signal Transducing physiology
Cytoskeletal Proteins physiology
Intestinal Mucosa metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 5
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 20209148
- Full Text :
- https://doi.org/10.1371/journal.pone.0009468