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Phosphatidylserine immobilization of lentivirus for localized gene transfer.
- Source :
-
Biomaterials [Biomaterials] 2010 May; Vol. 31 (15), pp. 4353-9. Date of Electronic Publication: 2010 Mar 04. - Publication Year :
- 2010
-
Abstract
- Localized and efficient gene transfer can be promoted by exploiting the interaction between the vector and biomaterial. Regulation of the vector-material interaction was investigated by capitalizing on the binding between lentivirus and phosphatidylserine (PS), a component of the plasma membrane. PS was incorporated into microspheres composed of the copolymers of lactide and glycolide (PLG) using an emulsion process. Increasing the weight ratio of PS to PLG led to a greater incorporation of PS. Lentivirus, but not adenovirus, associated with PS-PLG microspheres, and binding was specific to PS relative to PLG alone or PLG modified with phosphatidylcholine. Immobilized lentivirus produced large numbers of transduced cells, and increased transgene expression relative to virus alone. Microspheres were subsequently formed into porous tissue engineering scaffolds, with retention of lentivirus binding. Lentivirus immobilization resulted in long-term and localized expression within a subcutaneously implanted scaffold. Microspheres were also formed into multiple channel bridges for implantation into the spinal cord. Lentivirus delivery from the bridge produced maximal expression at the implant and a gradient of expression rostrally and caudally. This specific binding of lentiviral vectors to biomaterial scaffolds may provide a versatile tool for numerous applications in regenerative medicine or within model systems that investigate tissue development.<br /> (Copyright (c) 2010 Elsevier Ltd. All rights reserved.)
- Subjects :
- Adenoviridae genetics
Adenoviridae metabolism
Animals
Biocompatible Materials chemistry
Biocompatible Materials metabolism
Female
Materials Testing
Microspheres
Polyglactin 910 chemistry
Polyglactin 910 metabolism
Rats
Rats, Long-Evans
Spinal Cord metabolism
Spinal Cord pathology
Gene Transfer Techniques
Genetic Vectors genetics
Genetic Vectors metabolism
Lentivirus genetics
Lentivirus metabolism
Phosphatidylserines metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1878-5905
- Volume :
- 31
- Issue :
- 15
- Database :
- MEDLINE
- Journal :
- Biomaterials
- Publication Type :
- Academic Journal
- Accession number :
- 20206382
- Full Text :
- https://doi.org/10.1016/j.biomaterials.2010.02.013