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Digestibility and allergenicity assessment of enzymatically crosslinked beta-casein.
- Source :
-
Molecular nutrition & food research [Mol Nutr Food Res] 2010 Sep; Vol. 54 (9), pp. 1273-84. - Publication Year :
- 2010
-
Abstract
- Crosslinking enzymes are frequently used in bioprocessing of dairy products. The aim of this study was to examine the effects of enzymatic crosslinking on IgE binding, allergenicity and digestion stability of beta-casein (CN). beta-CN was crosslinked by transglutaminase, tyrosinase, mushroom tyrosinase/caffeic acid and laccase/caffeic acid. The IgE binding to beta-CN was compared in vitro by CAP inhibition assay, ELISA inhibition as well as ex vivo by basophil activation assay. Crosslinked CNs were digested by simulated gastric fluid for 15 and 60 min and obtained digests analyzed for their ability to inhibit IgE binding by CAP inhibition assay and SDS-PAGE. The ability of crosslinked CNs to activate basophils was significantly reduced in seven patients in the case of CN crosslinked by laccase and moderately reduced in the case of tyrosinase/caffeic acid crosslinked CN (in two cow's milk allergy patients tested with different allergen concentrations). The response to various crosslinked CNs differed individually among patients' sera tested by ELISA inhibition assay. The presence of caffeic acid hampered digestion by pepsin, and this effect was most pronounced for the tyrosinase/caffeic acid crosslinked CN. The laccase/caffeic acid and mushroom tyrosinase/caffeic acid had the highest potential in mitigating IgE binding and allergenicity of the beta-CN out of all investigated enzymes. The presence of a small phenolic compound also increased digestion stability of beta-CN.
- Subjects :
- Adolescent
Allergens chemistry
Basophil Degranulation Test
Caffeic Acids metabolism
Caseins chemistry
Child, Preschool
Enzyme-Linked Immunosorbent Assay
Food Handling methods
Humans
Immunoglobulin E immunology
Immunoglobulin E metabolism
Kinetics
Laccase metabolism
Milk Hypersensitivity blood
Monophenol Monooxygenase metabolism
Polymerization
Protein Hydrolysates chemistry
Protein Hydrolysates metabolism
Transglutaminases metabolism
Allergens immunology
Allergens metabolism
Caseins immunology
Caseins metabolism
Digestion
Milk Hypersensitivity immunology
Protein Hydrolysates immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1613-4133
- Volume :
- 54
- Issue :
- 9
- Database :
- MEDLINE
- Journal :
- Molecular nutrition & food research
- Publication Type :
- Academic Journal
- Accession number :
- 20205191
- Full Text :
- https://doi.org/10.1002/mnfr.200900184