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The cell-free protein synthesis system from wheat germ.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2010; Vol. 607, pp. 23-30. - Publication Year :
- 2010
-
Abstract
- The wheat-germ cell-free protein synthesis system had been one of the most efficient eukaryotic cell-free systems since it was first developed in 1964. However, radio-labeled amino acids had long been essential for detection of the products. Since the discovery of a method for prevention of the contamination by a protein synthesis inhibitor originated from endosperm, the wheat cell-free system has found a wide variety of applications in postgenomic high-throughput screening, structural biology, medicine, and so on. In this chapter, we describe a method for preparation of the cell-free extract and a standard protein synthesis method, as the methods for the applications are found in later chapters.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 607
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 20204845
- Full Text :
- https://doi.org/10.1007/978-1-60327-331-2_3