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A combined method for producing homogeneous glycoproteins with eukaryotic N-glycosylation.
- Source :
-
Nature chemical biology [Nat Chem Biol] 2010 Apr; Vol. 6 (4), pp. 264-6. Date of Electronic Publication: 2010 Feb 28. - Publication Year :
- 2010
-
Abstract
- We describe a new method for producing homogeneous eukaryotic N-glycoproteins. The method involves the engineering and functional transfer of the Campylobacter jejuni glycosylation machinery in Escherichia coli to express glycosylated proteins with the key GlcNAc-Asn linkage. The bacterial glycans were then trimmed and remodeled in vitro by enzymatic transglycosylation to fulfill a eukaryotic N-glycosylation. It provides a potentially general platform for producing eukaryotic N-glycoproteins.
Details
- Language :
- English
- ISSN :
- 1552-4469
- Volume :
- 6
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Nature chemical biology
- Publication Type :
- Academic Journal
- Accession number :
- 20190762
- Full Text :
- https://doi.org/10.1038/nchembio.314