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Protection against lethal Rift Valley fever virus (RVFV) infection in transgenic IFNAR(-/-) mice induced by different DNA vaccination regimens.
- Source :
-
Vaccine [Vaccine] 2010 Apr 09; Vol. 28 (17), pp. 2937-44. Date of Electronic Publication: 2010 Feb 25. - Publication Year :
- 2010
-
Abstract
- In this work, plasmid constructs encoding two different M segment ORFs, as well as the nucleoprotein N, have been used in different vaccination regimes to test protection against a RVFV-MP12 virus challenge in a transgenic mouse model with impaired interferon type I response (IFNAR(-/-)). We obtained dose dependent protection in animals immunized with a construct encoding both mature glycoproteins (pCMV-M4), whereas only partial protection in animals vaccinated with either N construct (pCMV-N) or a combination of both plasmids (pCMV-M4+pCMV-N). The protection elicited by the expression of the mature glycoproteins could be directly related to the induction of neutralizing antibodies against them. Interestingly, the combination of both vaccine constructs induced specific lymphoblast proliferation upon stimulation with a recombinant nucleoprotein.<br /> (Copyright 2010 Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Antibodies, Neutralizing blood
Antibodies, Viral blood
Cell Proliferation
Humans
Lymphocytes immunology
Mice
Mice, Knockout
Mice, Transgenic
Rift Valley Fever immunology
Rift Valley fever virus genetics
Survival Analysis
Vaccines, DNA genetics
Viral Proteins genetics
Viral Proteins immunology
Viral Vaccines genetics
Receptor, Interferon alpha-beta deficiency
Rift Valley Fever prevention & control
Rift Valley fever virus immunology
Vaccination methods
Vaccines, DNA immunology
Viral Vaccines immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2518
- Volume :
- 28
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 20188678
- Full Text :
- https://doi.org/10.1016/j.vaccine.2010.02.018