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The cag PAI is intact and functional but HP0521 varies significantly in Helicobacter pylori isolates from Malaysia and Singapore.
- Source :
-
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology [Eur J Clin Microbiol Infect Dis] 2010 Apr; Vol. 29 (4), pp. 439-51. Date of Electronic Publication: 2010 Feb 16. - Publication Year :
- 2010
-
Abstract
- Helicobacter pylori-related disease is at least partially attributable to the genotype of the infecting strain, particularly the presence of specific virulence factors. We investigated the prevalence of a novel combination of H. pylori virulence factors, including the cag pathogenicity island (PAI), and their association with severe disease in isolates from the three major ethnicities in Malaysia and Singapore, and evaluated whether the cag PAI was intact and functional in vitro. Polymerase chain reaction (PCR) was used to detect dupA, cagA, cagE, cagT, cagL and babA, and to type vacA, the EPIYA motifs, HP0521 alleles and oipA ON status in 159 H. pylori clinical isolates. Twenty-two strains were investigated for IL-8 induction and CagA translocation in vitro. The prevalence of cagA, cagE, cagL, cagT, babA, oipA ON and vacA s1 and i1 was >85%, irrespective of the disease state or ethnicity. The prevalence of dupA and the predominant HP0521 allele and EPIYA motif varied significantly with ethnicity (p < 0.05). A high prevalence of an intact cag PAI was found in all ethnic groups; however, no association was observed between any virulence factor and disease state. The novel association between the HP0521 alleles, EPIYA motifs and host ethnicity indicates that further studies to determine the function of this gene are important.
- Subjects :
- Adult
Antigens, Bacterial metabolism
Bacterial Proteins genetics
Bacterial Proteins metabolism
Cells, Cultured
Epithelial Cells microbiology
Helicobacter pylori classification
Humans
Interleukin-8 metabolism
Malaysia
Polymerase Chain Reaction
Protein Transport
Singapore
DNA, Bacterial genetics
Genetic Variation
Genomic Islands
Helicobacter Infections microbiology
Helicobacter pylori genetics
Helicobacter pylori isolation & purification
Virulence Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1435-4373
- Volume :
- 29
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 20157752
- Full Text :
- https://doi.org/10.1007/s10096-010-0881-7