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Monocyte and neutrophil isolation and migration assays.

Monocyte and neutrophil isolation and migration assays.

Authors :
Yona S
Hayhoe R
Avraham-Davidi I
Source :
Current protocols in immunology [Curr Protoc Immunol] 2010 Feb; Vol. Chapter 14, pp. 14.15.1-14.15.14.
Publication Year :
2010

Abstract

This unit describes methods for isolating mouse monocytes and neutrophils, as well as in vitro protocols for measuring cell migration and polarization. The method employed here for the isolation of naïve phagocytes overcomes many of the difficulties previously encountered concerning phagocyte activation. Three in vitro protocols are provided for the analysis of cell migration, one requiring no specialized equipment, one requiring the modified Boyden chamber, and the other employing a flow chamber, which measures cell adhesion, rolling, and migration. Finally, a method is provided for imaging polarized cells by confocal microscopy.

Details

Language :
English
ISSN :
1934-368X
Volume :
Chapter 14
Database :
MEDLINE
Journal :
Current protocols in immunology
Publication Type :
Academic Journal
Accession number :
20143313
Full Text :
https://doi.org/10.1002/0471142735.im1415s88