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Sp1 and KLF15 regulate basal transcription of the human LRP5 gene.
- Source :
-
BMC genetics [BMC Genet] 2010 Feb 08; Vol. 11, pp. 12. Date of Electronic Publication: 2010 Feb 08. - Publication Year :
- 2010
-
Abstract
- Background: LRP5, a member of the low density lipoprotein receptor superfamily, regulates diverse developmental processes in embryogenesis and maintains physiological homeostasis in adult organisms. However, how the expression of human LRP5 gene is regulated remains unclear.<br />Results: In order to characterize the transcriptional regulation of human LRP5 gene, we cloned the 5' flanking region and evaluated its transcriptional activity in a luciferase reporter system. We demonstrated that both KLF15 and Sp1 binding sites between -72 bp and -53 bp contribute to the transcriptional activation of human LRP5 promoter. Chromatin immunoprecipitation assay demonstrated that the ubiquitous transcription factors KLF15 and Sp1 bind to this region. Using Drosophila SL2 cells, we showed that KLF15 and Sp1 trans-activated the LRP5 promoter in a manner dependent on the presence of Sp1-binding and KLF15-binding motifs.<br />Conclusions: Both KLF15 and Sp1 binding sites contribute to the basal activity of human LRP5 promoter. This study provides the first insight into the mechanisms by which transcription of human LRP5 gene is regulated.
- Subjects :
- Animals
Base Sequence
Binding Sites
Cell Line, Tumor
Chromatin Immunoprecipitation
Cloning, Molecular
Drosophila genetics
Gene Expression Regulation
Humans
Low Density Lipoprotein Receptor-Related Protein-5
Molecular Sequence Data
Promoter Regions, Genetic
Kruppel-Like Transcription Factors genetics
LDL-Receptor Related Proteins genetics
Nuclear Proteins genetics
Sp1 Transcription Factor genetics
Transcriptional Activation
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2156
- Volume :
- 11
- Database :
- MEDLINE
- Journal :
- BMC genetics
- Publication Type :
- Academic Journal
- Accession number :
- 20141633
- Full Text :
- https://doi.org/10.1186/1471-2156-11-12