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A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454.

Authors :
Lennon NJ
Lintner RE
Anderson S
Alvarez P
Barry A
Brockman W
Daza R
Erlich RL
Giannoukos G
Green L
Hollinger A
Hoover CA
Jaffe DB
Juhn F
McCarthy D
Perrin D
Ponchner K
Powers TL
Rizzolo K
Robbins D
Ryan E
Russ C
Sparrow T
Stalker J
Steelman S
Weiand M
Zimmer A
Henn MR
Nusbaum C
Nicol R
Source :
Genome biology [Genome Biol] 2010; Vol. 11 (2), pp. R15. Date of Electronic Publication: 2010 Feb 05.
Publication Year :
2010

Abstract

We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and cleanup steps have been devised, eliminating major bottlenecks and significant sources of error. Using this methodology, one technician can create 96 sequence-ready 454 libraries in 2 days, a dramatic improvement over the standard method.

Details

Language :
English
ISSN :
1474-760X
Volume :
11
Issue :
2
Database :
MEDLINE
Journal :
Genome biology
Publication Type :
Academic Journal
Accession number :
20137071
Full Text :
https://doi.org/10.1186/gb-2010-11-2-r15