Back to Search
Start Over
A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454.
- Source :
-
Genome biology [Genome Biol] 2010; Vol. 11 (2), pp. R15. Date of Electronic Publication: 2010 Feb 05. - Publication Year :
- 2010
-
Abstract
- We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and cleanup steps have been devised, eliminating major bottlenecks and significant sources of error. Using this methodology, one technician can create 96 sequence-ready 454 libraries in 2 days, a dramatic improvement over the standard method.
Details
- Language :
- English
- ISSN :
- 1474-760X
- Volume :
- 11
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Genome biology
- Publication Type :
- Academic Journal
- Accession number :
- 20137071
- Full Text :
- https://doi.org/10.1186/gb-2010-11-2-r15