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Effect of insulin on oogenesis from mouse fetal germ cells in a serum-free 3D culture system.
Effect of insulin on oogenesis from mouse fetal germ cells in a serum-free 3D culture system.
- Source :
-
Reproductive biomedicine online [Reprod Biomed Online] 2010 Jan; Vol. 20 (1), pp. 11-25. Date of Electronic Publication: 2009 Nov 15. - Publication Year :
- 2010
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Abstract
- Continuous exposure of oocytes to elevated concentrations of insulin compromises embryonic developmental competence. However, the effects of insulin on oogenesis from fetal germ cells are unknown. The objective of this study was to assess the effect of continuous insulin exposure, with or without FSH, on oogenesis and follicular development. A simple and efficient method was established that could be used to obtain oocytes from pre-meiotic germ cells in 12.5days post-coitum (dpc) fetal mouse ovaries using a three-dimensional culture system with serum-free medium. Mouse 12.5dpc fetal ovaries were cultured for 14days with or without insulin/FSH. Low (0.2-1microg/ml) or high (5-20microg/ml) doses of insulin retarded oocyte growth in vitro. Insulin at 5microg/ml led to significant oocyte growth retardation (P<0.05), while FSH alleviated the deleterious effect of insulin. Most importantly, the proportion of secondary follicles at 12days post-culture in the presence of insulin was reduced significantly compared with controls (P<0.05). Expression levels of genes specific for ovarian cells, e.g. Cx37, Cx43, Scp3, Bax and FSHR, were significantly reduced when exposed to insulin during oogenesis (P<0.05). The data suggest that insulin has a profound detrimental effect on oogenesis and folliculogenesis in vitro.<br /> (Copyright (c) 2009 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All rights reserved.)
- Subjects :
- Animals
Cell Culture Techniques methods
Cell Differentiation drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Female
Follicle Stimulating Hormone pharmacology
Gene Expression drug effects
Meiotic Prophase I drug effects
Mice
Mice, Inbred Strains
Oocytes physiology
Ovum cytology
Pregnancy
Hypoglycemic Agents pharmacology
Insulin pharmacology
Oocytes drug effects
Oogenesis drug effects
Ovum drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 1472-6491
- Volume :
- 20
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Reproductive biomedicine online
- Publication Type :
- Academic Journal
- Accession number :
- 20129134
- Full Text :
- https://doi.org/10.1016/j.rbmo.2009.11.001