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Functional characterization of the copper-transporting P-type ATPase gene of Penicillium janthinellum strain GXCR.
- Source :
-
Journal of microbiology (Seoul, Korea) [J Microbiol] 2009 Dec; Vol. 47 (6), pp. 736-45. Date of Electronic Publication: 2010 Feb 04. - Publication Year :
- 2009
-
Abstract
- Copper (Cu)-transporting P-type ATPase (CTPA) genes have been documented to play an important role in resistance to heavy metals. However, our understanding of roles of CTPA genes of the filamentous fungi was based only on sequence similarity prediction before. In a previous study, we isolated a Penicillum janthinellum strain GXCR of higher tolerance to Cu (200 mM). In this study, we cloned the partial cDNA of CTPA gene, named PcpA, from the strain GXCR. Sequence alignment indicated that the cloned cDNA sequence has the highest identity (94.4%) with a predictive CTPA gene of Aspergillus clavatus. The PcpA-encoded protein, termed PcpA, has classical functional domains of CTPAs, and shows differences from reported CTPAs in some specific sequence motifs and transmembrane regions. Expression of the PcpA was induced by extracellular Cu, cadmium (Cd), and silver (Ag). PcpA RNA interference (RNAi) mutants with a reduced level of PcpA mRNA were more sensitive to Cu, iron, Cd, and Ag than the wild-type (WT) strain GXCR. When grown in the presence of Cu, iron, and Cd, intracellular Cu and iron contents in the PcpA RNAi mutant were significantly (P<0.05) lower than those in the WT; However, intracellular Cd content in the mutant was significantly (P<0.05) higher than that in the WT. Taken together, it can be concluded that the PcpA functions in Cu uptake and homeostasis, iron uptake, and Cd export from the cytosol to the extracytosol.
- Subjects :
- Aspergillus genetics
Cadmium metabolism
DNA, Complementary genetics
DNA, Complementary isolation & purification
DNA, Fungal chemistry
DNA, Fungal genetics
DNA, Fungal isolation & purification
Gene Expression Profiling
Gene Expression Regulation, Enzymologic
Gene Expression Regulation, Fungal
Gene Knockdown Techniques
Microbial Sensitivity Tests
Molecular Sequence Data
Protein Structure, Tertiary
Sequence Alignment
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Silver metabolism
Adenosine Triphosphatases metabolism
Copper metabolism
Penicillium enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1976-3794
- Volume :
- 47
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Journal of microbiology (Seoul, Korea)
- Publication Type :
- Academic Journal
- Accession number :
- 20127468
- Full Text :
- https://doi.org/10.1007/s12275-009-0074-1