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Substance P stimulates differentiation of mice osteoblast through up-regulating Osterix expression.
- Source :
-
Chinese journal of traumatology = Zhonghua chuang shang za zhi [Chin J Traumatol] 2010 Feb; Vol. 13 (1), pp. 46-50. - Publication Year :
- 2010
-
Abstract
- Objective: To investigate the molecular pathway of substance P (SP) to induce osteoblastic differentiation.<br />Methods: Mesenchymal stem cells were isolated and cultured. The cultures were divided into four groups with Group A (control group) cultured without any factors, Group B cultured with SP, Group C cultured with SP and SP receptor neurokinin-1 (NK1) antagonist, and Group D cultured with SP NK1 antagonist respectively to induce osteoblastic cells differentiation. Osterix gene expression was detected by reverse transcription-polymerase chain reaction (RT-PCR) for three times after 1-2 weeks of cultivation and the results were analyzed by one-way analysis of variance (ANOVA).<br />Results: The log phase of bone marrow stromal cells appeared at 4-6 days. ALP staining revealed that the majority of cells, more than 95%, were positive and small blue-purple granules were found in the cytoplasm. And Group B, treated with SP, showed a higher level of ALP activity than the other three groups. Meanwhile, RT-PCR found that Osterix expression in Group B was obviously up-regulated, compared with other groups. But Osterix expression in Group D had no remarkable differences, compared with the controls.<br />Conclusions: SP can up-regulate Osterix gene expression to stimulate differentiation of mesenchymal stem cells into osteoblastic cells at the final stage. The regulatory effect of SP on Osterix expression was dependant on SP NK1 receptors.
- Subjects :
- Alkaline Phosphatase analysis
Animals
Cell Differentiation drug effects
Gene Expression Regulation drug effects
Osteoblasts cytology
Rats
Rats, Wistar
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation
Osteoblasts drug effects
Substance P pharmacology
Transcription Factors genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1008-1275
- Volume :
- 13
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Chinese journal of traumatology = Zhonghua chuang shang za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 20109368