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Macrophage receptors for influenza A virus: role of the macrophage galactose-type lectin and mannose receptor in viral entry.
- Source :
-
Journal of virology [J Virol] 2010 Apr; Vol. 84 (8), pp. 3730-7. Date of Electronic Publication: 2010 Jan 27. - Publication Year :
- 2010
-
Abstract
- Although sialic acid has long been recognized as the primary receptor determinant for attachment of influenza virus to host cells, the specific receptor molecules that mediate viral entry are not known for any cell type. For the infection of murine macrophages by influenza virus, our earlier study indicated involvement of a C-type lectin, the macrophage mannose receptor (MMR), in this process. Here, we have used direct binding techniques to confirm and characterize the interaction of influenza virus with the MMR and to seek additional macrophage surface molecules that may have potential as receptors for viral entry. We identified the macrophage galactose-type lectin (MGL) as a second macrophage membrane C-type lectin that binds influenza virus and is known to be endocytic. Binding of influenza virus to MMR and MGL occurred independently of sialic acid through Ca(2+)-dependent recognition of viral glycans by the carbohydrate recognition domains of the two lectins; influenza virus also bound to the sialic acid on the MMR. Multivalent ligands of the MMR and MGL inhibited influenza virus infection of macrophages in a manner that correlated with expression of these receptors on different macrophage populations. Influenza virus strain A/PR/8/34, which is poorly glycosylated and infects macrophages poorly, was not recognized by the C-type lectin activity of either the MMR or the MGL. We conclude that lectin-mediated interactions of influenza virus with the MMR or the MGL are required for the endocytic uptake of the virus into macrophages, and these lectins can thus be considered secondary or coreceptors with sialic acid for infection of this cell type.
- Subjects :
- Animals
Calcium metabolism
Cations, Divalent metabolism
Cell Line
Cells, Cultured
Mannose Receptor
Mice
Mice, Inbred C57BL
N-Acetylneuraminic Acid metabolism
Asialoglycoproteins physiology
Influenza A Virus, H1N1 Subtype physiology
Influenza A Virus, H3N2 Subtype physiology
Lectins, C-Type physiology
Macrophages virology
Mannose-Binding Lectins physiology
Membrane Proteins physiology
Receptors, Cell Surface physiology
Receptors, Virus physiology
Virus Internalization
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5514
- Volume :
- 84
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 20106926
- Full Text :
- https://doi.org/10.1128/JVI.02148-09