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The transposon impala is activated by low temperatures: use of a controlled transposition system to identify genes critical for viability of Aspergillus fumigatus.
- Source :
-
Eukaryotic cell [Eukaryot Cell] 2010 Mar; Vol. 9 (3), pp. 438-48. Date of Electronic Publication: 2010 Jan 22. - Publication Year :
- 2010
-
Abstract
- Genes that are essential for viability represent potential targets for the development of anti-infective agents. However, relatively few have been determined in the filamentous fungal pathogen Aspergillus fumigatus. A novel solution employing parasexual genetics coupled with transposon mutagenesis using the Fusarium oxysporum transposon impala had previously enabled the identification of 20 essential genes from A. fumigatus; however, further use of this system required a better understanding of the mode of action of the transposon itself. Examination of a range of conditions indicated that impala is activated by prolonged exposure to low temperatures. This newly identified property was then harnessed to identify 96 loci that are critical for viability in A. fumigatus, including genes required for RNA metabolism, organelle organization, protein transport, ribosome biogenesis, and transcription, as well as a number of noncoding RNAs. A number of these genes represent potential targets for much-needed novel antifungal drugs.
- Subjects :
- Aspergillus fumigatus growth & development
Aspergillus nidulans genetics
Diploidy
Fusarium genetics
Gene Expression genetics
Haploidy
Kinetics
Mutagenesis, Insertional genetics
Promoter Regions, Genetic genetics
Reverse Transcriptase Polymerase Chain Reaction
Transformation, Genetic
Transposases genetics
Aspergillus fumigatus cytology
Aspergillus fumigatus genetics
Cold Temperature
DNA Transposable Elements genetics
Genes, Fungal genetics
Microbial Viability genetics
Subjects
Details
- Language :
- English
- ISSN :
- 1535-9786
- Volume :
- 9
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Eukaryotic cell
- Publication Type :
- Academic Journal
- Accession number :
- 20097738
- Full Text :
- https://doi.org/10.1128/EC.00324-09