Back to Search
Start Over
Adaptation of NS cells growth and differentiation to high-throughput screening-compatible plates.
- Source :
-
BMC neuroscience [BMC Neurosci] 2010 Jan 19; Vol. 11, pp. 7. Date of Electronic Publication: 2010 Jan 19. - Publication Year :
- 2010
-
Abstract
- Background: There is an urgent need of neuronal cell models to be applied to high-throughput screening settings while recapitulating physiological and/or pathological events occurring in the Central Nervous System (CNS). Stem cells offer a great opportunity in this direction since their self renewal capacity allows for large scale expansion. Protocols for directed differentiation also promise to generate populations of biochemically homogenous neuronal progenies. NS (Neural Stem) cells are a novel population of stem cells that undergo symmetric cell division in monolayer and chemically defined media, while remaining highly neurogenic.<br />Results: We report the full adaptation of the NS cell systems for their growth and neuronal differentiation to 96- and 384-well microplates. This optimized system has also been exploited in homogeneous and high-content assays.<br />Conclusions: Our results show that these mouse NS cells may be suitable for a series of applications in high-throughput format.
- Subjects :
- Animals
Apoptosis
Cell Differentiation
Cell Line
Cell Survival
Cyclic AMP metabolism
Mice
Mice, Inbred Strains
Neurogenesis
Oxidative Stress physiology
Receptors, GABA-A metabolism
Stem Cell Niche physiology
Time Factors
Adult Stem Cells physiology
Cell Culture Techniques instrumentation
Cell Culture Techniques methods
High-Throughput Screening Assays instrumentation
High-Throughput Screening Assays methods
Neurons physiology
Subjects
Details
- Language :
- English
- ISSN :
- 1471-2202
- Volume :
- 11
- Database :
- MEDLINE
- Journal :
- BMC neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 20085655
- Full Text :
- https://doi.org/10.1186/1471-2202-11-7