Back to Search
Start Over
Immunoregulatory properties of mouse limbal stem cells.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 2010 Feb 15; Vol. 184 (4), pp. 2124-9. Date of Electronic Publication: 2010 Jan 11. - Publication Year :
- 2010
-
Abstract
- Stem cells have been demonstrated in nearly all adult mammalian tissues and play a vital role in their physiological renewal and healing after injury. Due to their irreplaceable role in tissue repair, these cells had to develop mechanisms protecting them from deleterious inflammatory immune reactions and ensuring their increased resistance to various apoptosis-inducing agents. In this study, we demonstrate that a population of mouse limbal cells highly enriched for cells expressing markers and characteristics of limbal stem cells (LSCs) suppresses in a dose-dependent manner the proliferation of lymphocytes elicited by mitogens or TCR-triggering and significantly inhibits the production of proinflammatory cytokines by activated T cells. The suppression was mediated by soluble factor(s) and did not affect early cell activation. LSCs were even more suppressive than mesenchymal stem cells or natural regulatory T cells. In addition, the cells expressing markers and characteristics of LSC had significantly higher levels of mRNA for Fas ligand and for the antiapoptotic molecules Mcl-1, XIAP, and survivin than other limbal cell populations. LSCs were also more resistant to staurosporin-induced apoptotic cell death and to cell-mediated cytotoxic reaction than other limbal cells. Collectively, these results suggest that SC isolated from fresh adult limbal tissue possess immunomodulatory properties and inhibit proinflammatory immune reactions. Simultaneously, these cells express high levels of mRNA for antiapoptotic molecules, which can protect them against cell-mediated cytotoxic reactions and various apoptosis-inducing agents.
- Subjects :
- Animals
Cell Proliferation
Cell Separation methods
Cells, Cultured
Cytokines antagonists & inhibitors
Cytokines biosynthesis
Cytotoxicity Tests, Immunologic methods
Epithelium, Corneal chemistry
Female
Growth Inhibitors biosynthesis
Growth Inhibitors isolation & purification
Growth Inhibitors physiology
Immunologic Factors biosynthesis
Immunologic Factors isolation & purification
Immunosuppressive Agents metabolism
Immunosuppressive Agents pharmacology
Male
Mice
Mice, Inbred BALB C
Stem Cells chemistry
Stem Cells cytology
Epithelium, Corneal cytology
Epithelium, Corneal immunology
Immunologic Factors physiology
Immunosuppressive Agents isolation & purification
Stem Cells immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1550-6606
- Volume :
- 184
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 20065115
- Full Text :
- https://doi.org/10.4049/jimmunol.0903049