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Glycopeptides from epithelial cell mutants: temperature sensitive for the transformation phenotype.
- Source :
-
International journal of cancer [Int J Cancer] 1977 Nov 15; Vol. 20 (5), pp. 738-47. - Publication Year :
- 1977
-
Abstract
- Fucose-labelled glycopeptides obtained from the cell surfaces of normal and transformed epithelial cells were compared by co-chromatography on Sephadex G-50. The material obtained from epithelial cells transformed in vitro or from hepatoma cells in culture elutes earlier than the fucose-containing glycopeptides obtained from normal rat epithelial cells. A mutant (TS 223) of a transformed epithelial cell that is temperature-sensitive for maintenance of the transformed phenotype, varies in its Sephadex G-50 profile of cell surface glycopeptides when grown at the permissive (36 degrees C) or the non-permissive temperature (40 degrees C). When grown and labelled at 36 degrees C the gel filtration profile of the glycopeptides resembles that of transformed cells. At 40 degrees C there is an enrichment of later eluting glycopeptides. These differences are more striking in confluent-phase cultures than in log-phase culture. The differences are reversible following upward or downward shifts in growth temperature although there appears to be a lag of at least 6 h before the alteration can be demonstrated by these procedures.
Details
- Language :
- English
- ISSN :
- 0020-7136
- Volume :
- 20
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- International journal of cancer
- Publication Type :
- Academic Journal
- Accession number :
- 200573
- Full Text :
- https://doi.org/10.1002/ijc.2910200514