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Antimicrobial susceptibility testing using high surface-to-volume ratio microchannels.
- Source :
-
Analytical chemistry [Anal Chem] 2010 Feb 01; Vol. 82 (3), pp. 1012-9. - Publication Year :
- 2010
-
Abstract
- This study reports the use of microfluidics, which intrinsically has a large surface-to-volume ratio, toward rapid antimicrobial susceptibility testing at the point of care. By observing the growth of uropathogenic Escherichia coli in gas permeable polymeric microchannels with different dimensions, we demonstrate that the large surface-to-volume ratio of microfluidic systems facilitates rapid growth of bacteria. For microchannels with 250 microm or less in depth, the effective oxygenation can sustain the growth of E. coli to over 10(9) cfu/mL without external agitation or oxygenation, which eliminates the requirement of bulky instrumentation and facilitates rapid bacterial growth for antimicrobial susceptibility testing at the point of care. The applicability of microfluidic rapid antimicrobial susceptibility testing is demonstrated in culture media and in urine with clinical bacterial isolates that have different antimicrobial resistance profiles. The antimicrobial resistance pattern can be determined as rapidly as 2 h compared to days in standard clinical procedures facilitating diagnostics at the point of care.
- Subjects :
- Dimethylpolysiloxanes chemistry
Escherichia coli isolation & purification
Microbial Sensitivity Tests methods
Microfluidic Analytical Techniques methods
Oxygen chemistry
Surface Properties
Urinary Tract Infections microbiology
Anti-Bacterial Agents pharmacology
Escherichia coli drug effects
Microbial Sensitivity Tests instrumentation
Microfluidic Analytical Techniques instrumentation
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 82
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 20055494
- Full Text :
- https://doi.org/10.1021/ac9022764