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Fate specification and tissue-specific cell cycle control of the Caenorhabditis elegans intestine.
- Source :
-
Molecular biology of the cell [Mol Biol Cell] 2010 Mar 01; Vol. 21 (5), pp. 725-38. Date of Electronic Publication: 2010 Jan 06. - Publication Year :
- 2010
-
Abstract
- Coordination between cell fate specification and cell cycle control in multicellular organisms is essential to regulate cell numbers in tissues and organs during development, and its failure may lead to oncogenesis. In mammalian cells, as part of a general cell cycle checkpoint mechanism, the F-box protein beta-transducin repeat-containing protein (beta-TrCP) and the Skp1/Cul1/F-box complex control the periodic cell cycle fluctuations in abundance of the CDC25A and B phosphatases. Here, we find that the Caenorhabditis elegans beta-TrCP orthologue LIN-23 regulates a progressive decline of CDC-25.1 abundance over several embryonic cell cycles and specifies cell number of one tissue, the embryonic intestine. The negative regulation of CDC-25.1 abundance by LIN-23 may be developmentally controlled because CDC-25.1 accumulates over time within the developing germline, where LIN-23 is also present. Concurrent with the destabilization of CDC-25.1, LIN-23 displays a spatially dynamic behavior in the embryo, periodically entering a nuclear compartment where CDC-25.1 is abundant.
- Subjects :
- Animals
Caenorhabditis elegans Proteins metabolism
Cell Cycle
Cell Cycle Proteins metabolism
Cell Lineage
Cell Nucleus metabolism
F-Box Proteins metabolism
Immunohistochemistry methods
Microscopy, Confocal methods
Models, Biological
Models, Genetic
Phenotype
RNA Interference
cdc25 Phosphatases metabolism
Caenorhabditis elegans physiology
Gene Expression Regulation, Developmental
Intestines cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1939-4586
- Volume :
- 21
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Molecular biology of the cell
- Publication Type :
- Academic Journal
- Accession number :
- 20053685
- Full Text :
- https://doi.org/10.1091/mbc.E09-04-0268