Back to Search
Start Over
Epithelial induction of porcine suppressor of cytokine signaling 2 (SOCS2) gene expression in response to Entamoeba histolytica.
- Source :
-
Developmental and comparative immunology [Dev Comp Immunol] 2010 May; Vol. 34 (5), pp. 562-71. Date of Electronic Publication: 2010 Jan 06. - Publication Year :
- 2010
-
Abstract
- Suppressor of cytokine signaling (SOCS) proteins are key physiological regulators of both innate and adaptive immunity. These proteins belong to the three major classes of modulators of cytokines signaling. In the following article, we used porcine polarized intestinal cells to study early response to the protozoan, Entamoeba histolytica, and we identified by rapid amplification of cDNA ends (RACE) PCR porcine SOCS1, SOCS4, SOCS5 and SOCS6 encoding sequences. With more than 92% identity predicted porcine SOCS proteins are very similar to their human counterparts. Among SOCS transcripts, only SOCS2 mRNA was significantly induced in epithelial intestinal cells in response to the cytolytic activity of the parasite. The transcriptomic profile obtained after 3h of co-culture of polarized intestinal cells with E. histolytica was clearly oriented toward inflammation and the recruitment of neutrophils. These transcriptomic data have been normalized with accuracy by the utilisation of multiple validated reference genes. The analysis offers a first set of reference genes useful for future studies in porcine intestinal cells. Our data shed light on the understanding of the early response of polarized intestinal cells to E. histolytica and identified a potential involvement of SOCS2 in the parasite regulation of the host response.<br /> (Copyright (c) 2010 Elsevier Ltd. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Cell Movement genetics
Cell Movement immunology
Cells, Cultured
Entamoeba histolytica pathogenicity
Entamoebiasis genetics
Entamoebiasis metabolism
Gene Expression Profiling
Gene Expression Regulation immunology
Host-Parasite Interactions
Humans
Inflammation
Intestinal Mucosa immunology
Intestinal Mucosa microbiology
Intestinal Mucosa pathology
Molecular Sequence Data
Neutrophils immunology
Neutrophils metabolism
Neutrophils pathology
Sequence Homology, Amino Acid
Suppressor of Cytokine Signaling Proteins genetics
Suppressor of Cytokine Signaling Proteins immunology
Swine
Entamoeba histolytica immunology
Entamoebiasis immunology
Intestinal Mucosa metabolism
Suppressor of Cytokine Signaling Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1879-0089
- Volume :
- 34
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Developmental and comparative immunology
- Publication Type :
- Academic Journal
- Accession number :
- 20045027
- Full Text :
- https://doi.org/10.1016/j.dci.2009.12.017