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Preparation of biomolecule microstructures and microarrays by thiol-ene photoimmobilization.

Authors :
Weinrich D
Köhn M
Jonkheijm P
Westerlind U
Dehmelt L
Engelkamp H
Christianen PC
Kuhlmann J
Maan JC
Nüsse D
Schröder H
Wacker R
Voges E
Breinbauer R
Kunz H
Niemeyer CM
Waldmann H
Source :
Chembiochem : a European journal of chemical biology [Chembiochem] 2010 Jan 25; Vol. 11 (2), pp. 235-47.
Publication Year :
2010

Abstract

A mild, fast and flexible method for photoimmobilization of biomolecules based on the light-initiated thiol-ene reaction has been developed. After investigation and optimization of various surface materials, surface chemistries and reaction parameters, microstructures and microarrays of biotin, oligonucleotides, peptides, and MUC1 tandem repeat glycopeptides were prepared with this photoimmobilization method. Furthermore, MUC1 tandem repeat glycopeptide microarrays were successfully used to probe antibodies in mouse serum obtained from vaccinated mice. Dimensions of biomolecule microstructures were shown to be freely controllable through photolithographic techniques, and features down to 5 microm in size covering an area of up to 75x25 mm were created. Use of a confocal laser microscope with a UV laser as UV-light source enabled further reduction of biotin feature size opening access to nanostructured biochips.

Details

Language :
English
ISSN :
1439-7633
Volume :
11
Issue :
2
Database :
MEDLINE
Journal :
Chembiochem : a European journal of chemical biology
Publication Type :
Academic Journal
Accession number :
20043307
Full Text :
https://doi.org/10.1002/cbic.200900559