Back to Search
Start Over
Cytotoxic effects of ultra-diluted remedies on breast cancer cells.
- Source :
-
International journal of oncology [Int J Oncol] 2010 Feb; Vol. 36 (2), pp. 395-403. - Publication Year :
- 2010
-
Abstract
- The use of ultra-diluted natural products in the management of disease and treatment of cancer has generated a lot of interest and controversy. We conducted an in vitro study to determine if products prescribed by a clinic in India have any effect on breast cancer cell lines. We studied four ultra-diluted remedies (Carcinosin, Phytolacca, Conium and Thuja) against two human breast adenocarcinoma cell lines (MCF-7 and MDA-MB-231) and a cell line derived from immortalized normal human mammary epithelial cells (HMLE). The remedies exerted preferential cytotoxic effects against the two breast cancer cell lines, causing cell cycle delay/arrest and apoptosis. These effects were accompanied by altered expression of the cell cycle regulatory proteins, including downregulation of phosphorylated Rb and upregulation of the CDK inhibitor p27, which were likely responsible for the cell cycle delay/arrest as well as induction of the apoptotic cascade that manifested in the activation of caspase 7 and cleavage of PARP in the treated cells. The findings demonstrate biological activity of these natural products when presented at ultra-diluted doses. Further in-depth studies with additional cell lines and animal models are warranted to explore the clinical applicability of these agents.
- Subjects :
- Apoptosis drug effects
Blotting, Western
Breast Neoplasms metabolism
Cell Cycle drug effects
Cell Line, Tumor
Cell Proliferation drug effects
Cell Separation
Cell Survival drug effects
Chromatography, High Pressure Liquid
Conium chemistry
Female
Flow Cytometry
Gene Expression drug effects
Humans
In Situ Hybridization, Fluorescence
India
Phytolacca chemistry
Telomere drug effects
Thuja chemistry
Antineoplastic Agents pharmacology
Breast Neoplasms drug therapy
Homeopathy methods
Phytotherapy methods
Subjects
Details
- Language :
- English
- ISSN :
- 1791-2423
- Volume :
- 36
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- International journal of oncology
- Publication Type :
- Academic Journal
- Accession number :
- 20043074