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Sphingosine 1-phosphate signaling is involved in skeletal muscle regeneration.
- Source :
-
American journal of physiology. Cell physiology [Am J Physiol Cell Physiol] 2010 Mar; Vol. 298 (3), pp. C550-8. Date of Electronic Publication: 2009 Dec 30. - Publication Year :
- 2010
-
Abstract
- Sphingosine 1-phosphate (S1P) is a bioactive lipid known to control cell growth that was recently shown to act as a trophic factor for skeletal muscle, reducing the progress of denervation atrophy. The aim of this work was to investigate whether S1P is involved in skeletal muscle fiber recovery (regeneration) after myotoxic injury induced by bupivacaine. The postnatal ability of skeletal muscle to grow and regenerate is dependent on resident stem cells called satellite cells. Immunofluorescence analysis demonstrated that S1P-specific receptors S1P(1) and S1P(3) are expressed by quiescent satellite cells. Soleus muscles undergoing regeneration following injury induced by intramuscular injection of bupivacaine exhibited enhanced expression of S1P(1) receptor, while S1P(3) expression progressively decreased to adult levels. S1P(2) receptor was absent in quiescent cells but was transiently expressed in the early regenerating phases only. Administration of S1P (50 microM) at the moment of myotoxic injury caused a significant increase of the mean cross-sectional area of regenerating fibers in both rat and mouse. In separate experiments designed to test the trophic effects of S1P, neutralization of endogenous circulating S1P by intraperitoneal administration of anti-S1P antibody attenuated fiber growth. Use of selective modulators of S1P receptors indicated that S1P(1) receptor negatively and S1P(3) receptor positively modulate the early phases of regeneration, whereas S1P(2) receptor appears to be less important. The present results show that S1P signaling participates in the regenerative processes of skeletal muscle.
- Subjects :
- Animals
Bupivacaine
Cell Membrane metabolism
Cell Proliferation
Cells, Cultured
Disease Models, Animal
Injections, Intramuscular
Lysophospholipids administration & dosage
Male
Mice
Mice, Inbred C57BL
Muscle, Skeletal drug effects
Muscle, Skeletal physiopathology
Muscular Diseases chemically induced
Muscular Diseases physiopathology
Rats
Rats, Wistar
Receptors, Lysosphingolipid drug effects
Receptors, Lysosphingolipid metabolism
Satellite Cells, Skeletal Muscle drug effects
Sphingosine administration & dosage
Sphingosine metabolism
Time Factors
Lysophospholipids metabolism
Muscle Development drug effects
Muscle, Skeletal metabolism
Muscular Diseases metabolism
Regeneration drug effects
Satellite Cells, Skeletal Muscle metabolism
Signal Transduction drug effects
Sphingosine analogs & derivatives
Subjects
Details
- Language :
- English
- ISSN :
- 1522-1563
- Volume :
- 298
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- American journal of physiology. Cell physiology
- Publication Type :
- Academic Journal
- Accession number :
- 20042733
- Full Text :
- https://doi.org/10.1152/ajpcell.00072.2009