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A robust and high-throughput Cre reporting and characterization system for the whole mouse brain.
- Source :
-
Nature neuroscience [Nat Neurosci] 2010 Jan; Vol. 13 (1), pp. 133-40. Date of Electronic Publication: 2009 Dec 20. - Publication Year :
- 2010
-
Abstract
- The Cre/lox system is widely used in mice to achieve cell-type-specific gene expression. However, a strong and universally responding system to express genes under Cre control is still lacking. We have generated a set of Cre reporter mice with strong, ubiquitous expression of fluorescent proteins of different spectra. The robust native fluorescence of these reporters enables direct visualization of fine dendritic structures and axonal projections of the labeled neurons, which is useful in mapping neuronal circuitry, imaging and tracking specific cell populations in vivo. Using these reporters and a high-throughput in situ hybridization platform, we are systematically profiling Cre-directed gene expression throughout the mouse brain in several Cre-driver lines, including new Cre lines targeting different cell types in the cortex. Our expression data are displayed in a public online database to help researchers assess the utility of various Cre-driver lines for cell-type-specific genetic manipulation.
- Subjects :
- Animals
Bacterial Proteins genetics
Brain cytology
Dendrites metabolism
Gene Transfer Techniques
Integrases physiology
Luminescent Proteins genetics
Mice
Mice, Inbred C57BL
Mice, Transgenic
Microscopy, Confocal
Neurons cytology
Promoter Regions, Genetic
Regulatory Sequences, Nucleic Acid
Tamoxifen pharmacology
Tissue Distribution
Viral Proteins genetics
Viral Proteins metabolism
Brain metabolism
Integrases genetics
Recombination, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1546-1726
- Volume :
- 13
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Nature neuroscience
- Publication Type :
- Academic Journal
- Accession number :
- 20023653
- Full Text :
- https://doi.org/10.1038/nn.2467