Back to Search
Start Over
[Evaluation of immunohistochemistry staining and cytologic diagnosis by using cell block sections prepared with effusion fluid cytology specimens].
- Source :
-
Zhonghua bing li xue za zhi = Chinese journal of pathology [Zhonghua Bing Li Xue Za Zhi] 2009 Aug; Vol. 38 (8), pp. 547-50. - Publication Year :
- 2009
-
Abstract
- Objective: To study the values of immunohistochemistry staining and cytological diagnosis by using cell block sections prepared with the effusion fluid cytology specimens.<br />Methods: Ninety-nine effusion cytology specimens with the diagnoses of reactive mesothelial hyperplasia, atypical cells and metastatic carcinoma were enrolled into the study. The cytospin preparations/smears, cell block sections and immunohistochemical study were performed and correlated with the clinical findings and follow-up data.<br />Results: Amongst the 99 cases studied, the percentage with positive diagnosis using cytospin preparations/smears was 68.7% (68/99). The percentages with negative and equivocal diagnoses were 16.2% (16/99) and 15.1% (15/99), respectively. As for cell block sections, the percentages were 71.7% (71/99), 16.2% (16/99) and 12.1% (12/99), respectively. On the other hands, the percentages became 76.8% (76/99), 20.2% (20/99) and 3.0% (3/99), respectively, when coupled with immunohistochemical findings. The overall percentages of positive, negative and equivocal diagnoses were 77.8% (77/99), 17.2% (17/99) and 5.0% (5/99), respectively, upon clinicopathologic correlation. The difference between cytospin preparations/smears and cell block sections was not statistically significant (P > 0.05). When coupled with immunohistochemical findings or clinicopathologic correlation, the difference in rates of equivocal diagnosis however carried statistical significance (P < 0.05). The false-negative rate of immunohistochemical study applied on cell block sections was 1.0% (1/99).<br />Conclusions: Immunohistochemistry, when applied on cell block sections, is useful in delineation of the primary origins of the tumor cells in effusion fluid cytology specimens. Combination of morphologic examination, immunohistochemical findings and clinicopathologic correlation can further improve the rate of positive diagnosis.
- Subjects :
- Adult
Aged
Aged, 80 and over
Ascites metabolism
Ascitic Fluid metabolism
CA-125 Antigen metabolism
Carcinoembryonic Antigen metabolism
Cytodiagnosis
Female
Gastrointestinal Neoplasms metabolism
Humans
Immunohistochemistry
Lung Neoplasms metabolism
Male
Membrane Proteins metabolism
Middle Aged
Ovarian Neoplasms metabolism
Pericardial Effusion metabolism
Pericardial Effusion pathology
Pleural Effusion metabolism
Pleural Effusion pathology
Pleural Effusion, Malignant metabolism
Pleural Effusion, Malignant pathology
Young Adult
Ascites pathology
Ascitic Fluid pathology
Gastrointestinal Neoplasms pathology
Lung Neoplasms pathology
Ovarian Neoplasms pathology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0529-5807
- Volume :
- 38
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Zhonghua bing li xue za zhi = Chinese journal of pathology
- Publication Type :
- Academic Journal
- Accession number :
- 20021967