Repetitive extragenic palindromic PCR for study of Streptococcus mutans diversity and transmission in human populations.

Authors :: Moser SA
Mitchell SC
Ruby JD
Momeni S
Osgood RC
Whiddon J
Childers NK
Source :: Journal of clinical microbiology [J Clin Microbiol] 2010 Feb; Vol. 48 (2), pp. 599-602. Date of Electronic Publication: 2009 Dec 09.
Publication Year :: 2010
Abstract: Pulsed-field gel electrophoresis (PFGE) is considered the "gold standard" for molecular epidemiological study. Repetitive extragenic palindromic PCR (rep-PCR) is less time-consuming and more suitable for analyzing large numbers of bacterial strains in human populations. PFGE and rep-PCR provide comparable genotyping results for investigating Streptococcus mutans diversity and transmission.

Subjects :: Child
Child, Preschool
Cluster Analysis
Electrophoresis, Gel, Pulsed-Field
Female
Genotype
Humans
Infant
Male
Molecular Epidemiology methods
Polymorphism, Genetic
Streptococcal Infections microbiology
Streptococcal Infections transmission
Streptococcus mutans isolation & purification
Bacterial Typing Techniques methods
DNA Fingerprinting methods
Polymerase Chain Reaction methods
Streptococcus mutans classification
Streptococcus mutans genetics

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