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[Comparative characterization of catalytic properties of mitochondrial and cytoplasmic forms of NAD-dependent malate dehydrogenase from the rat liver at norm and in toxic hepatitis].

Authors :
Mikhaĭlova EV
Popova TN
Safonova OA
Source :
Biomeditsinskaia khimiia [Biomed Khim] 2009 Jul-Aug; Vol. 55 (4), pp. 489-99.
Publication Year :
2009

Abstract

The purification and comparative characterization of some catalytic properties of liver mitochondrial and cytosolic NAD-dependent malate dehydrogenase (NAD-MDH; EC 1.1.1.37) from normal rats and rats with experimental toxic hepatitis (ETH) have been carried out. It has been found that there are some differences in catalytic and regulatory properties of liver NAD-MDH from control animals and rats with ETH. It has been shown that Fe2+ and Cu2+ ions inhibit the enzyme, and the inhibition degree is different at norm and under toxic hepatitis. Ca2+ ions insignificantly activate cytosolic NAD-MDH under pathology and do not influence the mitochondrial isoform.

Details

Language :
Russian
ISSN :
2310-6972
Volume :
55
Issue :
4
Database :
MEDLINE
Journal :
Biomeditsinskaia khimiia
Publication Type :
Academic Journal
Accession number :
20000126