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[Comparative characterization of catalytic properties of mitochondrial and cytoplasmic forms of NAD-dependent malate dehydrogenase from the rat liver at norm and in toxic hepatitis].
- Source :
-
Biomeditsinskaia khimiia [Biomed Khim] 2009 Jul-Aug; Vol. 55 (4), pp. 489-99. - Publication Year :
- 2009
-
Abstract
- The purification and comparative characterization of some catalytic properties of liver mitochondrial and cytosolic NAD-dependent malate dehydrogenase (NAD-MDH; EC 1.1.1.37) from normal rats and rats with experimental toxic hepatitis (ETH) have been carried out. It has been found that there are some differences in catalytic and regulatory properties of liver NAD-MDH from control animals and rats with ETH. It has been shown that Fe2+ and Cu2+ ions inhibit the enzyme, and the inhibition degree is different at norm and under toxic hepatitis. Ca2+ ions insignificantly activate cytosolic NAD-MDH under pathology and do not influence the mitochondrial isoform.
- Subjects :
- Animals
Calcium chemistry
Catalysis
Cations, Divalent
Copper chemistry
Enzyme Activation
Hydrogen-Ion Concentration
Iron chemistry
Kinetics
Malate Dehydrogenase antagonists & inhibitors
Malate Dehydrogenase chemistry
Male
Oxaloacetic Acid chemistry
Rats
Chemical and Drug Induced Liver Injury enzymology
Cytoplasm enzymology
Liver enzymology
Malate Dehydrogenase isolation & purification
Mitochondria, Liver enzymology
Subjects
Details
- Language :
- Russian
- ISSN :
- 2310-6972
- Volume :
- 55
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Biomeditsinskaia khimiia
- Publication Type :
- Academic Journal
- Accession number :
- 20000126