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Antibody complementarity-determining regions (CDRs): a bridge between adaptive and innate immunity.
- Source :
-
PloS one [PLoS One] 2009 Dec 04; Vol. 4 (12), pp. e8187. Date of Electronic Publication: 2009 Dec 04. - Publication Year :
- 2009
-
Abstract
- Background: It has been documented that, independently from the specificity of the native antibody (Ab) for a given antigen (Ag), complementarity determining regions (CDR)-related peptides may display differential antimicrobial, antiviral and antitumor activities.<br />Methodology/principal Findings: In this study we demonstrate that a synthetic peptide with sequence identical to V(H)CDR3 of a mouse monoclonal Ab (mAb) specific for difucosyl human blood group A is easily taken up by macrophages with subsequent stimulation of: i) proinflammatory cytokine production; ii) PI3K-Akt pathway and iii) TLR-4 expression. Significantly, V(H)CDR3 exerts therapeutic effect against systemic candidiasis without possessing direct candidacidal properties.<br />Conclusions/significance: These results open a new scenario about the possibility that, beyond the half life of immunoglobulins, Ab fragments may effectively influence the antiinfective cellular immune response in a way reminiscent of regulatory peptides of innate immunity.
- Subjects :
- Adaptive Immunity drug effects
Animals
Antifungal Agents pharmacology
Candida albicans drug effects
Candida albicans immunology
Candidiasis immunology
Candidiasis pathology
Complementarity Determining Regions pharmacology
Enzyme Activation drug effects
Humans
Immunity, Innate drug effects
Immunoglobulin Heavy Chains immunology
Immunoglobulin Variable Region immunology
Immunomodulation drug effects
Macrophages, Peritoneal drug effects
Macrophages, Peritoneal enzymology
Macrophages, Peritoneal immunology
Mice
Neutrophils drug effects
Neutrophils immunology
Peptides immunology
Peptides pharmacology
Proto-Oncogene Proteins c-akt metabolism
Survival Analysis
Toll-Like Receptor 4 genetics
Tumor Necrosis Factor-alpha biosynthesis
Up-Regulation drug effects
Adaptive Immunity immunology
Complementarity Determining Regions immunology
Immunity, Innate immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1932-6203
- Volume :
- 4
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- PloS one
- Publication Type :
- Academic Journal
- Accession number :
- 19997599
- Full Text :
- https://doi.org/10.1371/journal.pone.0008187