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Defective polymorphonuclear leukocyte formyl peptide receptor(s) in juvenile periodontitis.
- Source :
-
The Journal of clinical investigation [J Clin Invest] 1991 Mar; Vol. 87 (3), pp. 971-6. - Publication Year :
- 1991
-
Abstract
- Juvenile periodontitis (JP) is a disease characterized by severe gingival infections. PMN from some JP patients exhibit abnormal chemotactic responsiveness when challenged with the synthetic formyl peptide, FMLP. While investigating PMN function in JP, we found a patient in whom abnormal PMN chemotactic responses to FMLP were associated with a defective population of PMN formyl peptide receptor(s) (FPR). JP PMN failed to respond chemotactically when challenged with FMLP, but exhibited normal chemotactic responses upon exposure to purified human C5a. Furthermore, JP PMN were capable of degranulating and generating superoxide anion radicals as well as normal PMN upon exposure to FMLP. Binding studies demonstrated that JP PMN had a diminution in the number of high-affinity FPR. Studies in which FPR was radiolabeled by chemical cross-linking demonstrated that JP PMN FPR exhibited the same molecular weight and N-linked glycosylation as normal PMN FPR. JP PMN FPR, however, was more resistant to papain cleavage than normal PMN FPR. Autoradiograms obtained from 2D-PAGE of normal and JP PMN FPR demonstrated decreased amounts of FPR isoforms in JP PMN.
- Subjects :
- Adult
Affinity Labels
Cell Degranulation drug effects
Chemotaxis, Leukocyte
Complement C5a pharmacology
Electrophoresis, Gel, Two-Dimensional
Female
Humans
Male
N-Formylmethionine Leucyl-Phenylalanine pharmacology
Peptide Mapping
Receptors, Formyl Peptide
Receptors, Immunologic chemistry
Aggressive Periodontitis physiopathology
N-Formylmethionine Leucyl-Phenylalanine metabolism
Neutrophils physiology
Receptors, Immunologic physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9738
- Volume :
- 87
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Journal of clinical investigation
- Publication Type :
- Academic Journal
- Accession number :
- 1999504
- Full Text :
- https://doi.org/10.1172/JCI115105