Generation of a zinc finger protein ZPR1 mutant that constitutively interacted with translation elongation factor 1alpha.

Authors :: Yanaka N
Kaseda Y
Tanaka A
Nogusa Y
Sumiyoshi N
Kato N
Source :: Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2009 Dec; Vol. 73 (12), pp. 2809-11. Date of Electronic Publication: 2009 Dec 07.
Publication Year :: 2009
Abstract: Zinc finger protein ZPR1 (ZPR1) binds to eukaryotic translation elongation factor 1alpha (eEF1alpha) in response to growth stimuli, and is also involved in transcription and cell cycle regulation. In this study, we characterized the interaction of ZPR1 and eEF1alpha and generated a ZPR1 mutant that constitutively interacted with eEF1alpha. ZPR1-DeltaA (Delta193-246) bound to eEF1alpha independently of Zn(2+) in vivo. This study indicates that ZPR1-DeltaA (Delta193-246) is a useful tool to provide structural insights into ZPR1 and to investigate the biological significance of the interaction between ZPR1 and eEF1alpha.

Subjects :: Animals
Cell Line
Humans
Intracellular Signaling Peptides and Proteins
Mice
Protein Binding
Carrier Proteins genetics
Carrier Proteins metabolism
Mutant Proteins genetics
Mutant Proteins metabolism
Mutation
Peptide Elongation Factor 1 metabolism

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