[Investigation on the relationship among histamine, tryptase and beta-hexosaminidase in the process of mast cell degranulation.].

Aim: To investigate the relationship among histamine, tryptase and beta-hexosaminidase in the process of mast cell release and search for the suitable index to describe mast cell degranulation.
Methods: Mast cell degranulation was performed in vitro, in which histamine, tryptase and beta-hexosaminidase are detected by spectrophotometry.
Results: The concordance was found to exit among the three mediums, furthermore the study discloses that more similarity was found between tryptase and beta-hexosaminidase. Histamine was difficult and inconstant to be detected.
Conclusion: Tryptase and beta-hexosaminidase are more suitable and accurately to reflect mast cell degranulation in vitro than histamine. Tryptase is the excellent index to detect mast cell degranulation in vitro.