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Environmental mutagens that induce the adaptive response to alkylating agents in Escherichia coli.
- Source :
-
Carcinogenesis [Carcinogenesis] 1991 Feb; Vol. 12 (2), pp. 263-8. - Publication Year :
- 1991
-
Abstract
- Many microorganisms exhibit an adaptive response to mutagenic alkylation damage. In Escherichia coli the response is regulated by the inducible Ada protein. A sensitive immunoassay employing two anti-Ada monoclonal antibodies has been developed here to monitor low levels of induction of the Ada protein. This protein was detected in non-induced E. coli which contained an average of two molecules of Ada per cell. The occurrence of the adaptive response in bacteria signals the existence of an ecological niche in which cells are exposed to direct-acting methylating compounds, but the structure and identity of these agents are unknown. Using the immunoassay to search for possible candidates, a number of methylating agents and precursors of such agents have been investigated. Carbamyl phosphate and methylamine yield N-methylurea, which reacts subsequently with nitrite to generate the strong inducer N-methyl-N-nitrosourea. The antibiotic streptozotocin also is a potent inducer of the adaptive response. Moreover, the abundant environmental mutagen methyl chloride acts as an inducer.
- Subjects :
- Adaptation, Physiological drug effects
Anti-Bacterial Agents pharmacology
Antibodies, Monoclonal immunology
Bacterial Proteins immunology
Bacterial Proteins metabolism
Creatinine metabolism
DNA Repair
Escherichia coli drug effects
Escherichia coli enzymology
Hydrogen-Ion Concentration
Immunoblotting
Intracellular Fluid metabolism
Methyl Chloride pharmacology
Methylnitronitrosoguanidine pharmacology
Methylnitrosourea pharmacology
Methyltransferases metabolism
Methylurea Compounds metabolism
O(6)-Methylguanine-DNA Methyltransferase
Transcription Factors
Alkylating Agents pharmacology
Carcinogens, Environmental pharmacology
Escherichia coli physiology
Escherichia coli Proteins
Mutagens pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0143-3334
- Volume :
- 12
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Carcinogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 1995192
- Full Text :
- https://doi.org/10.1093/carcin/12.2.263