Back to Search
Start Over
Intranasal immunization with a proteoliposome-derived cochleate containing recombinant gD protein confers protective immunity against genital herpes in mice.
- Source :
-
Vaccine [Vaccine] 2010 Feb 03; Vol. 28 (5), pp. 1193-200. Date of Electronic Publication: 2009 Nov 27. - Publication Year :
- 2010
-
Abstract
- The purpose of this study was to investigate the potential of intranasal (IN) immunization with Neisseria meningitides B proteoliposome (AFPL1) and AFPL1-derived cochleate (AFCo1), containing glycoprotein D (gD) of herpes simplex virus type 2 (HSV-2) for induction of protective immunity against genital herpes infection in mice. We could show that IN immunization with both AFPL1 and AFCo1 containing gD induced gD-specific IgG antibody and lymphoproliferative responses. However, IFN-gamma response could only be detected in CD4(+) splenic cells and genital lymph node cells of the AFCo1gD immunized mice upon recall antigen stimulation in vitro. Importantly, IN immunization with AFCo1gD could elicit a complete protection against an otherwise lethal vaginal challenge with HSV-2, while the AFPL1gD immunized mice were only partially protected. Further, we could show that the IFN-gamma response and protective immunity observed after IN immunization with AFCo1gD are mediated via the adaptor molecule myeloid differentiation factor 88. These data may have implications for the development of a mucosal vaccine against genital herpes.<br /> ((c) 2009 Elsevier Ltd. All rights reserved.)
- Subjects :
- Administration, Intranasal
Animals
Antibodies, Viral immunology
Antigens, Bacterial immunology
Antigens, Bacterial pharmacology
CD4-Positive T-Lymphocytes immunology
Female
Herpes Genitalis genetics
Herpes Genitalis immunology
Immunization
Immunoglobulin G immunology
Interferon-gamma immunology
Liposomes
Mice
Mice, Knockout
Myeloid Differentiation Factor 88 genetics
Myeloid Differentiation Factor 88 immunology
Neisseria meningitidis, Serogroup B immunology
Recombinant Proteins genetics
Recombinant Proteins immunology
Recombinant Proteins pharmacology
Viral Envelope Proteins genetics
Herpes Genitalis prevention & control
Herpesvirus 2, Human immunology
Viral Envelope Proteins immunology
Viral Envelope Proteins pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1873-2518
- Volume :
- 28
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 19945418
- Full Text :
- https://doi.org/10.1016/j.vaccine.2009.11.035