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Expression of leptin receptor gene in developing and adult zebrafish.
- Source :
-
General and comparative endocrinology [Gen Comp Endocrinol] 2010 Apr 01; Vol. 166 (2), pp. 346-55. Date of Electronic Publication: 2009 Nov 24. - Publication Year :
- 2010
-
Abstract
- Interactions of leptin and leptin receptors play crucial roles during animal development and regulation of appetite and energy balance. In this study we analyzed expression pattern of a zebrafish leptin receptor gene in both developing and adult zebrafish using in situ hybridization and Q-PCR methods. Zebrafish leptin receptor message (lepr) was detected in all embryonic and larval stages examined, and in adult zebrafish. In embryonic zebrafish, lepr was mainly expressed in the notochord. As development proceeded, lepr expression in the notochord decreased, while its expression in several other tissues, including the trunk muscles and gut, became evident. In both larval and adult brains, large lepr expressing cells were detected in similar regions of the hindbrain. In adult zebrafish, lepr expression was also observed in several other brain regions including the hypothalamic lateral tuberal nucleus, the fish homolog of the arcuate nucleus. Q-PCR experiments confirmed lepr expression in the adult fish brain, and also showed lepr expression in several adult tissues including liver, muscle and gonads. Our results showed that lepr expression was both spatially and temporally regulated.<br /> ((c) 2009 Elsevier Inc. All rights reserved.)
- Subjects :
- Amino Acid Sequence
Animals
Brain metabolism
Gene Expression Regulation
Gonads metabolism
In Situ Hybridization
Larva metabolism
Liver metabolism
Molecular Sequence Data
Muscles metabolism
Notochord metabolism
Organ Specificity
Receptors, Leptin chemistry
Sequence Alignment
Zebrafish embryology
Gene Expression
Receptors, Leptin genetics
Zebrafish growth & development
Zebrafish metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1095-6840
- Volume :
- 166
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- General and comparative endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 19941865
- Full Text :
- https://doi.org/10.1016/j.ygcen.2009.11.015