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Anti-L-NGFR and -CD34 monoclonal antibodies identify multipotent mesenchymal stem cells in human adipose tissue.
- Source :
-
Stem cells and development [Stem Cells Dev] 2010 Jun; Vol. 19 (6), pp. 915-25. - Publication Year :
- 2010
-
Abstract
- Stem cells hold great promise in tissue engineering for repairing tissues damaged by disease or injury. Mesenchymal stem cells (MSCs) are multipotent cells able to proliferate and differentiate into multiple mesodermal tissues such as bone, cartilage, muscle, tendon, and fat. We have previously reported that the low-affinity nerve growth factor receptor (L-NGFR or CD271) defines a subset of cells with high proliferative, clonogenic, and multipotential differentiation ability in adult bone marrow (BM). It has been recently shown that adipose tissue is an alternative source of adult multipotent stem cells and human adipose-derived stem cells, selected by plastic adherence (PA hASCs), have been extensively characterized for their functional potentials in vitro. In this study, immunoselected L-NGFR(+) and CD34(+) subpopulations have been analyzed and compared with the PA hASCs. Phenotypic profile of freshly purified subpopulations showed an enrichment in the expression of some stem cell markers; indeed, a great percentage of L-NGFR(+) cells co-expressed CD34 and CD117 antigens, whereas the endothelial-committed progenitor markers KDR and P1H12 were mainly expressed on CD34(+) cells. Differently from PA hASCs, the immunoseparated fractions showed high increments in cell proliferation, and the fibroblast colony-forming activity (CFU-F) was maintained throughout the time of culture. Furthermore, the immunoselected populations showed a greater differentiative potential toward adipocytes, osteoblasts, and chondrocyte-like cells, compared to PA hASCs. Our data suggest that both CD34(+) and L-NGFR(+) hASCs can be considered alternative candidates for tissue engineering and regenerative medicine applications.
- Subjects :
- Adipogenesis
Adult
Biomarkers metabolism
Cell Differentiation
Cell Membrane metabolism
Cell Proliferation
Colony-Forming Units Assay
Extracellular Matrix metabolism
Female
Glycosaminoglycans metabolism
Hematopoietic Stem Cells cytology
Hematopoietic Stem Cells metabolism
Humans
Immunophenotyping
Mesenchymal Stem Cells metabolism
Multipotent Stem Cells metabolism
Subcellular Fractions metabolism
Adipose Tissue cytology
Antibodies, Monoclonal immunology
Antigens, CD34 immunology
Mesenchymal Stem Cells cytology
Multipotent Stem Cells cytology
Receptor, Nerve Growth Factor immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1557-8534
- Volume :
- 19
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Stem cells and development
- Publication Type :
- Academic Journal
- Accession number :
- 19929314
- Full Text :
- https://doi.org/10.1089/scd.2009.0408