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Histone h3 exerts a key function in mitotic checkpoint control.

Histone h3 exerts a key function in mitotic checkpoint control.

Authors :
Luo J
Xu X
Hall H
Hyland EM
Boeke JD
Hazbun T
Kuo MH
Source :
Molecular and cellular biology [Mol Cell Biol] 2010 Jan; Vol. 30 (2), pp. 537-49. Date of Electronic Publication: 2009 Nov 16.
Publication Year :
2010

Abstract

It has been firmly established that many interphase nuclear functions, including transcriptional regulation, are regulated by chromatin and histones. How mitotic progression and quality control might be influenced by histones is less well characterized. We show that histone H3 plays a crucial role in activating the spindle assembly checkpoint in response to a defect in mitosis. Prior to anaphase, all chromosomes must attach to spindles emanating from the opposite spindle pole bodies. The tension between sister chromatids generated by the poleward pulling force is an integral part of chromosome biorientation. Lack of tension due to erroneous attachment activates the spindle assembly checkpoint, which corrects the mistakes and ensures segregation fidelity. A histone H3 mutation impairs the ability of yeast cells to activate the checkpoint in a tensionless crisis, leading to missegregation and aneuploidy. The defects in tension sensing result directly from an attenuated H3-Sgo1p interaction essential for pericentric recruitment of Sgo1p. Reinstating the pericentric enrichment of Sgo1p alleviates the mitotic defects. Histone H3, and hence the chromatin, is thus a key factor transmitting the tension status to the spindle assembly checkpoint.

Details

Language :
English
ISSN :
1098-5549
Volume :
30
Issue :
2
Database :
MEDLINE
Journal :
Molecular and cellular biology
Publication Type :
Academic Journal
Accession number :
19917722
Full Text :
https://doi.org/10.1128/MCB.00980-09