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Histone h3 exerts a key function in mitotic checkpoint control.
Histone h3 exerts a key function in mitotic checkpoint control.
- Source :
-
Molecular and cellular biology [Mol Cell Biol] 2010 Jan; Vol. 30 (2), pp. 537-49. Date of Electronic Publication: 2009 Nov 16. - Publication Year :
- 2010
-
Abstract
- It has been firmly established that many interphase nuclear functions, including transcriptional regulation, are regulated by chromatin and histones. How mitotic progression and quality control might be influenced by histones is less well characterized. We show that histone H3 plays a crucial role in activating the spindle assembly checkpoint in response to a defect in mitosis. Prior to anaphase, all chromosomes must attach to spindles emanating from the opposite spindle pole bodies. The tension between sister chromatids generated by the poleward pulling force is an integral part of chromosome biorientation. Lack of tension due to erroneous attachment activates the spindle assembly checkpoint, which corrects the mistakes and ensures segregation fidelity. A histone H3 mutation impairs the ability of yeast cells to activate the checkpoint in a tensionless crisis, leading to missegregation and aneuploidy. The defects in tension sensing result directly from an attenuated H3-Sgo1p interaction essential for pericentric recruitment of Sgo1p. Reinstating the pericentric enrichment of Sgo1p alleviates the mitotic defects. Histone H3, and hence the chromatin, is thus a key factor transmitting the tension status to the spindle assembly checkpoint.
- Subjects :
- Alleles
Anaphase genetics
Anaphase physiology
Cell Cycle Proteins genetics
Chromosomal Instability
Chromosome Segregation
Histones chemistry
Histones genetics
Mutation genetics
Mutation physiology
Saccharomyces cerevisiae metabolism
Saccharomyces cerevisiae Proteins genetics
Spindle Apparatus genetics
Cell Cycle Proteins metabolism
Histones metabolism
Mitosis
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins metabolism
Spindle Apparatus metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1098-5549
- Volume :
- 30
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Molecular and cellular biology
- Publication Type :
- Academic Journal
- Accession number :
- 19917722
- Full Text :
- https://doi.org/10.1128/MCB.00980-09