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Fusaric acid as a novel proton-affinitive derivatizing reagent for highly sensitive quantification of hydroxysteroids by LC-ESI-MS/MS.

Authors :
Yamashita K
Yamazaki K
Komatsu S
Numazawa M
Source :
Journal of the American Society for Mass Spectrometry [J Am Soc Mass Spectrom] 2010 Feb; Vol. 21 (2), pp. 249-53. Date of Electronic Publication: 2009 Nov 14.
Publication Year :
2010

Abstract

A highly sensitive derivatization method for liquid chromatography (LC)-electrospray ionization (ESI) tandem mass spectrometry of dehydroepiandrosterone (DHEA), testosterone (T), pregnenolone (P5), and 17alpha-OH-pregnenolone (17-OHP5) was developed based on the use of fusaric acid as a reagent. DHEA, P5, and 17-OHP5 were rapidly and quantitatively converted to the 3-fusarate esters by treatment with fusaric acid and 2-methyl-6-nitrobenzoic anhydride. The positive ESI-mass spectra of the fusarate esters of each steroid were dominated by the appearance of [M + H](+) as base peaks. The fusarate derivatization of these steroids showed 17.6-fold (DHEA), 11.9-fold (P5), 3.3-fold (17-OHP5), and 1.8-fold (T) higher sensitivity to those of the corresponding picolinate derivatives in LC-selected reaction monitoring.<br /> (2010 American Society for Mass Spectrometry. Published by Elsevier Inc. All rights reserved.)

Details

Language :
English
ISSN :
1879-1123
Volume :
21
Issue :
2
Database :
MEDLINE
Journal :
Journal of the American Society for Mass Spectrometry
Publication Type :
Academic Journal
Accession number :
19914845
Full Text :
https://doi.org/10.1016/j.jasms.2009.10.008