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Studies on the immune response and preparation of antibodies against a large panel of conjugated neurotransmitters and biogenic amines: specific polyclonal antibody response and tolerance.
- Source :
-
Journal of neurochemistry [J Neurochem] 2010 Feb; Vol. 112 (3), pp. 829-41. Date of Electronic Publication: 2009 Nov 13. - Publication Year :
- 2010
-
Abstract
- We described the production and characterization of antibodies against three important groups of neuro-active haptens, e.g., neurotransmitters and biogenic amines. First, from the tryptophane metabolic pathway: tryptamine, serotonin, 5-hydroxy-indole acetic acid, and melatonin. Secondly, the tyrosine metabolic pathway: tyramine, dopamine, dihydroxyphenyl acetic acid, and norepinephrine. Thirdly, antibodies against excitatory and inhibitory neurotransmitters: glycine, glutamate, glutamine, and GABA. Immunogenic conjugates were prepared after linking haptens to carrier proteins. Most antibodies displayed high specificity against corresponding neuro-active haptens conjugated in vitro and in situ in biological specimens, but not to closely related conjugated metabolites, precursors, pharmaceuticals, agonists, antagonists, or free neuro-active haptens. Conjugated norepinephrine was highly tolerant in different animal species and produced incidentally a short specific antibody response.
- Subjects :
- Animals
Antibodies metabolism
Antibody Specificity immunology
Biogenic Amines metabolism
Cross Reactions immunology
Enzyme-Linked Immunosorbent Assay methods
Haptens immunology
Immunization methods
Neurotransmitter Agents metabolism
Rabbits
Antibodies immunology
Antibody Formation physiology
Biogenic Amines immunology
Neurotransmitter Agents immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1471-4159
- Volume :
- 112
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 19912471
- Full Text :
- https://doi.org/10.1111/j.1471-4159.2009.06492.x