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Inhibition of human retinal pigment epithelial cell attachment, spreading, and migration by the human lectin galectin-1.
- Source :
-
Molecular vision [Mol Vis] 2009 Oct 23; Vol. 15, pp. 2162-73. Date of Electronic Publication: 2009 Oct 23. - Publication Year :
- 2009
-
Abstract
- Purpose: Adhesion and migration of dislocated retinal pigment epithelial (RPE) cells are initial steps in the pathogenesis of proliferative vitreoretinopathy (PVR). The role of the endogenous lectin, galectin-1, in attachment, spreading, and migration of human RPE cells was investigated from a therapeutic perspective.<br />Methods: Human RPE cells were treated with galectin-1 concentrations that ranged 0-250 microg/ml. Cell viability was tested by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT) assay. Galectin-1 binding to the RPE cells was investigated by immunocytochemistry. Attachment of RPE cells was assessed on 96-well plates coated with laminin, or fibronectin, or galectin-1, or the glycoprotein-lectin combinations and subsequent MTT-testing. RPE migration in the absence or presence of galectin-1 on the respective substrates was tested using a modified Boyden chamber assay with platelet derived growth factor (PDGF)-BB as the chemoattractant. Cellular spreading was characterized by cytoplasmic halo formation of RPE cells after three hours in contact with the surface coating.<br />Results: Galectin-1 bound to the cell surface. Binding could be inhibited by a beta-galactoside. MTT assays revealed no toxicity within control limits for the concentration range tested. When added to the medium, galectin-1 dose-dependently inhibited RPE cell attachment, spreading, and migration by more than 70%, irrespective of the substratum tested. When coated onto the plastic surface, galectin-1 alone impaired spreading and migration of RPE cells, and reduced attachment to and migration on fibronectin by up to 80%.<br />Conclusions: Galectin-1 inhibits RPE cell attachment, migration, and spreading in vitro with no apparent cytotoxicity. This activity of the endogenous effector deserves consideration as a potential therapeutic agent for the prevention of PVR.
- Subjects :
- Adult
Cell Adhesion drug effects
Cell Membrane drug effects
Cell Membrane metabolism
Cell Shape drug effects
Cell Survival drug effects
Cells, Cultured
Epithelial Cells cytology
Epithelial Cells drug effects
Epithelial Cells metabolism
Female
Fibronectins pharmacology
Galectin 1 toxicity
Humans
Laminin pharmacology
Male
Middle Aged
Pigment Epithelium of Eye drug effects
Protein Binding drug effects
Solubility drug effects
Cell Movement drug effects
Galectin 1 pharmacology
Pigment Epithelium of Eye cytology
Subjects
Details
- Language :
- English
- ISSN :
- 1090-0535
- Volume :
- 15
- Database :
- MEDLINE
- Journal :
- Molecular vision
- Publication Type :
- Academic Journal
- Accession number :
- 19898636