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Ghrelin receptor expression in lymphocytes isolated from adult cystic fibrosis patients.

Authors :
Cohen RI
Chandra S
Koenig S
Tsang D
Wilson D
McCloskey T
Source :
Respiration; international review of thoracic diseases [Respiration] 2010; Vol. 79 (2), pp. 141-6. Date of Electronic Publication: 2009 Oct 29.
Publication Year :
2010

Abstract

Background: To explore mechanisms of weight loss in cystic fibrosis (CF), we studied ghrelin receptor expression on isolated lymphocytes from CF subjects with different body mass indices (BMIs). Eating behavior is influenced by hormone peptides such as ghrelin, a potent appetite stimulator. However, studies on ghrelin plasma levels in CF showed it to be increased in cachectic subjects, the expected physiological response.<br />Objectives: (1) To compare ghrelin receptor expression between clinically stable CF subjects with normal BMI, CF subjects with cachexia and healthy controls. (2) To investigate ghrelin receptor expression in the same CF subjects before and after treatment for an acute exacerbation.<br />Methods: Lymphocytes were isolated from CF patients with normal BMI and low BMI and from controls. Ghrelin receptor quantification was determined via flow cytometry. Body composition was determined by bioelectrical impedance, and plasma levels of ghrelin, TNF-alpha, IL-1 and IL-6 were determined.<br />Results: CF subjects with low BMI had increased inflammation evidenced by increased plasma cytokines and showed decreased lymphocytic ghrelin receptor expression. Ghrelin receptor expression in the CF group with normal BMI was similar to controls; it decreased during an acute exacerbation associated with weight loss and returned to baseline following treatment and recovery of the weight loss.<br />Conclusions: Differences exist in ghrelin receptor expression in lymphocytes isolated from stable CF patients with different BMIs. These changes may be due to a disordered pathological response to weight loss.<br /> (Copyright 2009 S. Karger AG, Basel.)

Details

Language :
English
ISSN :
1423-0356
Volume :
79
Issue :
2
Database :
MEDLINE
Journal :
Respiration; international review of thoracic diseases
Publication Type :
Academic Journal
Accession number :
19887765
Full Text :
https://doi.org/10.1159/000254486