[Anaphylactic reactions in WZS minipig orally induced by glycinin].

Objective: To explore the suitable dosage and observation period of anaphylactic reactions in WZS minipigs induced by glycinin (11S).
Methods: Twelve 45-day-old WZS minipigs were randomly divided into three groups (control group, 4% 11S group and 8% 11S group, n = 4), which were respectively orally sensitized (0 - 10d) and orally challenged (16 - 18d, 31d) with diets containing 0%, 4% 11S or 8% 11S soybean proteins to induce anaphylactic reactions. Clinical symptoms, skin prick reactions, serum IgG, serum IgE, histamine, mast cell in the small intestine were observed or measured.
Results: Diarrhea and positive skin prick reactions at different degrees were observed in 11S groups. Compared with the control group, serum IgG, IgE and histamine levels of 4% 11S group at 19d and 32d were significantly higher (P < 0.05), and reached the peak when 11S groups were orally challenged at 19d. The jejunum histamine level was negatively correlated with the mast cell numbers in the jejunum mucosa and sub-mucosa (P < 0.05). Compared with the control, the mast cell number in the jejunum sub-mucosa of 4% 11S group was decreased (P < 0.05).
Conclusion: The WZS minipig allergic reaction orally induced by glycinin could be type I hypersensitivity mediated by IgE, reaction induced by 4% dosage glycinin could be more strong than that of 8% dosage, and stimulating phase could be a suitable observation period for anaphylactic reactions which could be related with the activation of mast cells in the small intestine.