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Agrobacterium-tumefaciens-mediated transformation of antifungal-lipopeptide-producing fungus Coleophoma empetri F-11899.
- Source :
-
Current genetics [Curr Genet] 2009 Dec; Vol. 55 (6), pp. 623-30. Date of Electronic Publication: 2009 Oct 30. - Publication Year :
- 2009
-
Abstract
- The filamentous fungus Coleophoma empetri F-11899 produces an echinocandin-like compound FR901379, the original source for micafungin which is prescribed to treat deep-seated mycoses. Despite its industrial importance, no genetic information on C. empetri F-11899 is currently available. To characterize FR901379 biosynthetic genes by insertional mutagenesis and to improve the compound production genetically, Agrobacterium tumefaciens-mediated transformation (ATMT) was attempted to make genetic manipulation possible in this strain. The optimum conditions for ATMT of C. empetri were determined for the cell density of bacteria, time period of co-cultivation and types of filters in co-cultivation. Using the established ATMT method, the hygromycin B resistant gene was successfully transferred into the genome of C. empetri F-11899 and stably maintained even after a serial passage. Some of these results will be applicable for ATMT of various filamentous fungi.
- Subjects :
- Antifungal Agents
DNA, Fungal isolation & purification
Escherichia coli Proteins genetics
Genetic Vectors genetics
Hygromycin B pharmacology
Mutagenesis, Insertional
Mycology methods
Phosphotransferases (Alcohol Group Acceptor) genetics
Saccharomycetales drug effects
Saccharomycetales metabolism
Agrobacterium tumefaciens genetics
DNA, Fungal genetics
Fungal Proteins genetics
Genes, Fungal
Peptides, Cyclic biosynthesis
Saccharomycetales genetics
Transformation, Genetic
Subjects
Details
- Language :
- English
- ISSN :
- 1432-0983
- Volume :
- 55
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Current genetics
- Publication Type :
- Academic Journal
- Accession number :
- 19876630
- Full Text :
- https://doi.org/10.1007/s00294-009-0275-5