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Apoptin enhances radiation-induced cell death in poorly responding head and neck squamous cell carcinoma cells.
- Source :
-
Basic & clinical pharmacology & toxicology [Basic Clin Pharmacol Toxicol] 2010 Feb; Vol. 106 (2), pp. 130-4. Date of Electronic Publication: 2009 Oct 27. - Publication Year :
- 2010
-
Abstract
- Treatment of head and neck cancers is still rather poor and worldwide new treatment options are sought. Sensitizing radioresistant tumours by combining irradiation with other therapeutics to induce apoptosis are widely investigated. We examined whether chicken anaemia virus-derived apoptin protein would have a beneficial effect on irradiation of radiosensitive SCC61 and radioresistant SQD9 human head and neck squamous carcinoma cell lines. In both cell lines, concurrent exposure to irradiation and apoptin resulted in analysed mitochondrial cytochrome c release and in cleavage of caspase-3, whereas irradiation alone of SQD9 cells under identical conditions did not. Moreover, in comparison with the irradiation, only the synchronized treatment of apoptin and irradiation resulted in increased cell death in especially the radioresistant SQD9 cells, as measured by means of a colony survival assay. Our data reveal that apoptin treatment represents an effective way for enhancing radiotherapy of tumours responding poorly to radiotherapy.
- Subjects :
- Carcinoma, Squamous Cell pathology
Carcinoma, Squamous Cell radiotherapy
Caspase 3 drug effects
Caspase 3 metabolism
Caspase 3 radiation effects
Cell Death drug effects
Cell Death radiation effects
Cell Line, Tumor
Combined Modality Therapy
Cytochromes c drug effects
Cytochromes c metabolism
Cytochromes c radiation effects
Head and Neck Neoplasms pathology
Head and Neck Neoplasms radiotherapy
Humans
Mitochondria drug effects
Mitochondria metabolism
Mitochondria radiation effects
Capsid Proteins pharmacology
Carcinoma, Squamous Cell drug therapy
Head and Neck Neoplasms drug therapy
Radiation-Sensitizing Agents pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1742-7843
- Volume :
- 106
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Basic & clinical pharmacology & toxicology
- Publication Type :
- Academic Journal
- Accession number :
- 19874285
- Full Text :
- https://doi.org/10.1111/j.1742-7843.2009.00482.x