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The recombination activating gene-1 (RAG-1) transcript is present in the murine central nervous system.
- Source :
-
Cell [Cell] 1991 Jan 11; Vol. 64 (1), pp. 189-200. - Publication Year :
- 1991
-
Abstract
- The recombination activating genes, RAG-1 and RAG-2, are likely to encode components of the V(D)J site-specific recombination machinery. We report here the detection of low levels of the RAG-1 transcript in the murine central nervous system by polymerase chain reaction, in situ hybridization, and Northern blot analyses. In contrast, an authentic RAG-2 transcript could not be detected reproducibly in the central nervous system. The RAG-1 transcript was found to be widespread in embryonic and postnatal neurons, with transcription being most apparent in regions of the postnatal brain with a high neuronal cell density (the cerebellum and the hippocampal formation). The results suggest that RAG-1 functions in neurons, where its role might be to recombine elements of the neuronal genome site-specifically, or to prevent detrimental alterations of the genome in these long-lived cells.
- Subjects :
- Aging
Animals
Base Sequence
Brain cytology
Brain embryology
Brain growth & development
Cell Division drug effects
Cell Line
Chromosome Mapping
Cytarabine pharmacology
Mice
Mice, Inbred BALB C
Molecular Sequence Data
Nucleic Acid Hybridization
Oligonucleotide Probes
Organ Specificity
Polymerase Chain Reaction
RNA Probes
Teratoma
Tretinoin pharmacology
Brain metabolism
Genes
Neurons metabolism
Recombination, Genetic
Transcription, Genetic drug effects
Subjects
Details
- Language :
- English
- ISSN :
- 0092-8674
- Volume :
- 64
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Cell
- Publication Type :
- Academic Journal
- Accession number :
- 1986864
- Full Text :
- https://doi.org/10.1016/0092-8674(91)90220-s