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Multiple blood-proteins approach for early-response exposure assessment using an in vivo murine radiation model.
- Source :
-
International journal of radiation biology [Int J Radiat Biol] 2009; Vol. 85 (10), pp. 837-50. - Publication Year :
- 2009
-
Abstract
- Purpose: The aim was to evaluate the utility of multiple blood-protein biomarkers for early-response assessment of radiation exposure using a murine radiation model system.<br />Material and Methods: BALB/c male mice (8-10 weeks old) were exposed to whole-body 60Co gamma-rays (10 cGy min(-1)) over a broad dose range (0-7 Gy). Blood protein biomarkers (i.e., Growth Arrest and DNA Damage Inducible Gene 45 or GADD45alpha, interleukin 6 or IL-6, and serum amyloid A or SAA) were measured by enzyme linked immunosorbent assay (ELISA) at 4, 24, 48, and 72 h after total-body irradiation (TBI).<br />Results: Time- and dose-dependent increases in the protein targets were observed. The use of multiple protein targets was evaluated using multiple linear regression analysis to provide dose-response calibration curves for dose assessment. Multivariate discriminant analysis demonstrated enhanced dose-dependent separation of irradiated animals from control as the number of biomarkers increased.<br />Conclusions: Results from this study represent a proof-of-concept for multiple blood-proteins biodosimetry approach. It was demonstrated for the first time that protein expression profile could be developed not only to assess radiation exposure in male BALB/c mice but also to distinguish the level of radiation exposure, ranging from 1-7 Gy.
- Subjects :
- Acute Radiation Syndrome blood
Acute Radiation Syndrome diagnosis
Animals
Biomarkers blood
Cell Cycle Proteins blood
Disease Models, Animal
Dose-Response Relationship, Radiation
Gamma Rays adverse effects
Humans
Interleukin-6 blood
Linear Models
Male
Mice
Mice, Inbred BALB C
Multivariate Analysis
Nuclear Proteins blood
Radioactive Hazard Release
Risk Assessment
Serum Amyloid A Protein metabolism
Whole-Body Irradiation adverse effects
Blood Proteins metabolism
Radiation Injuries, Experimental blood
Subjects
Details
- Language :
- English
- ISSN :
- 1362-3095
- Volume :
- 85
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- International journal of radiation biology
- Publication Type :
- Academic Journal
- Accession number :
- 19863200