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Multiple blood-proteins approach for early-response exposure assessment using an in vivo murine radiation model.

Authors :
Ossetrova NI
Blakely WF
Source :
International journal of radiation biology [Int J Radiat Biol] 2009; Vol. 85 (10), pp. 837-50.
Publication Year :
2009

Abstract

Purpose: The aim was to evaluate the utility of multiple blood-protein biomarkers for early-response assessment of radiation exposure using a murine radiation model system.<br />Material and Methods: BALB/c male mice (8-10 weeks old) were exposed to whole-body 60Co gamma-rays (10 cGy min(-1)) over a broad dose range (0-7 Gy). Blood protein biomarkers (i.e., Growth Arrest and DNA Damage Inducible Gene 45 or GADD45alpha, interleukin 6 or IL-6, and serum amyloid A or SAA) were measured by enzyme linked immunosorbent assay (ELISA) at 4, 24, 48, and 72 h after total-body irradiation (TBI).<br />Results: Time- and dose-dependent increases in the protein targets were observed. The use of multiple protein targets was evaluated using multiple linear regression analysis to provide dose-response calibration curves for dose assessment. Multivariate discriminant analysis demonstrated enhanced dose-dependent separation of irradiated animals from control as the number of biomarkers increased.<br />Conclusions: Results from this study represent a proof-of-concept for multiple blood-proteins biodosimetry approach. It was demonstrated for the first time that protein expression profile could be developed not only to assess radiation exposure in male BALB/c mice but also to distinguish the level of radiation exposure, ranging from 1-7 Gy.

Details

Language :
English
ISSN :
1362-3095
Volume :
85
Issue :
10
Database :
MEDLINE
Journal :
International journal of radiation biology
Publication Type :
Academic Journal
Accession number :
19863200