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Cytotoxic xanthone constituents of the stem bark of Garcinia mangostana (mangosteen).
- Source :
-
Journal of natural products [J Nat Prod] 2009 Nov; Vol. 72 (11), pp. 2028-31. - Publication Year :
- 2009
-
Abstract
- Bioassay-guided fractionation of a chloroform-soluble extract of Garcinia mangostana stem bark, using the HT-29 human colon cancer cell line and an enzyme-based ELISA NF-kappaB assay, led to the isolation of a new xanthone, 11-hydroxy-3-O-methyl-1-isomangostin (1). The structure of 1 was elucidated by spectroscopic data analysis. In addition, 10 other known compounds, 11-hydroxy-1-isomangostin (2), 11alpha-mangostanin (3), 3-isomangostin (4), alpha-mangostin (5), beta-mangostin (6), garcinone D (7), 9-hydroxycalabaxanthone (8), 8-deoxygartanin (9), gartanin (10), and cratoxyxanthone (11), were isolated. Compounds 4-8 exhibited cytotoxicity against the HT-29 cell line with ED50 values of 4.9, 1.7, 1.7, 2.3, and 9.1 microM, respectively. In an ELISA NF-kappaB assay, compounds 5-7, 9, and 10 inhibited p65 activation with IC50 values of 15.9, 12.1, 3.2, 11.3, and 19.0 microM, respectively, and 6 showed p50 inhibitory activity with an IC50 value of 7.5 microM. Alpha-mangostin (5) was further tested in an in vivo hollow fiber assay, using HT-29, LNCaP, and MCF-7 cells, but it was found to be inactive at the highest dose tested (20 mg/kg).
- Subjects :
- Antineoplastic Agents, Phytogenic chemistry
Drug Screening Assays, Antitumor
HT29 Cells
Humans
Indonesia
Models, Biological
Xanthones chemistry
Antineoplastic Agents, Phytogenic isolation & purification
Antineoplastic Agents, Phytogenic pharmacology
Garcinia mangostana chemistry
Plants, Medicinal chemistry
Xanthones isolation & purification
Xanthones pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1520-6025
- Volume :
- 72
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Journal of natural products
- Publication Type :
- Academic Journal
- Accession number :
- 19839614
- Full Text :
- https://doi.org/10.1021/np900517h